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anti-HSV-1-gD mouse monoclonal, EBS-I-042, purified

EBS-I-042 reacts with envelope glycoprotein D (gD) which is sufficient to facilitate fusion of herpes simplex to target cells.
Cat. No.: 691639
Quantity:  1 mL (100 µg/ml)
No. of experiments:  ≤ 2000 (IHC, 50 µl/slide)

Delivery Time: usually 5-10 working days

$481.10
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a kappa
Clone EBS-I-042
Immunogen HSV-1 isolate
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS with 0.02 % sodium azide
Storage store at 2-8°C
No. of experiments ≤ 2000 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity HSV-1 (negative with HSV-2)

Applications

Tested applications Tested dilutions
ELISA 1:1-1:100000 (0.001-100 µg/ml)
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml)

Background

Details

Membrane fusion is mediated by envelope glycoproteins for enveloped viruses like herpes simplex. Four of at least 10 viral glycoproteins are necessary and sufficient to facilitate fusion of herpes simplex to target cells. These four glycoproteins include glycoprotein B (gB), glycoprotein D (gD), glycoprotein H (gH) and glycoprotein L (gL). Fusion is dependent upon the expression of a gD receptor on target cell membranes.

Positive control: HSV-1 preparations and HSV-1 infected cells.

Reference

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