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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG2a kappa |
| Clone | EBS-I-042 |
| Immunogen | HSV-1 isolate |
| Purification | affinity chromatography |
| Conjugate | unconjugated |
| Formulation | PBS with 0.02 % sodium azide |
| Storage | store at 2-8°C |
| No. of experiments | ≤ 2000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | HSV-1 (negative with HSV-2) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | 1:1-1:100000 (0.001-100 µg/ml) |
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:100-1:200 (0.5-1.0 µg/ml) |
| Immunohistochemistry (IHC) - frozen | 1:50-1:100 (1-2 µg/ml) |
| Immunohistochemistry (IHC) - paraffin | 1:50-1:100 (1-2 µg/ml) |
Background
Details
Membrane fusion is mediated by envelope glycoproteins for enveloped viruses like herpes simplex. Four of at least 10 viral glycoproteins are necessary and sufficient to facilitate fusion of herpes simplex to target cells. These four glycoproteins include glycoprotein B (gB), glycoprotein D (gD), glycoprotein H (gH) and glycoprotein L (gL). Fusion is dependent upon the expression of a gD receptor on target cell membranes.
Positive control: HSV-1 preparations and HSV-1 infected cells.
Reference
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