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anti-Keratin 4,5,6,8,10,13,18 mouse monoclonal, C-11, purified

C11 reacts with keratins : 4,5,6,8,10,13 and 18.
Cat. No.: 691544
Quantity:  1 mL (100 µg/ml)

Delivery Time: usually 5-10 working days

$425.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1 kappa
Clone C-11
Immunogen cytoskeleton preparation of human A431 carcinoma cell line
Purification affinity chromatography
Conjugate unconjugated
Formulation PBS with 0.02 % sodium azide
Storage store at 2-8°C
Intended use Research use only
Tested species reactivity cat, chicken, dog, ferret, goat, guinea pig, human, monkey, mouse, rabbit, rat, xenopus

Applications

Tested applications Tested dilutions
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0,1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0,2% saponin or digitonin for 15 min, at 4°C
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/ml PBS recommended)
Western Blot (WB) 1:100 (1 µg/ml)

Background

Details

C11 reacts with keratins : 4,5,6,8,10,13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Positive control: A431 cells, skin, lung.

Reference

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