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WB with anti-K10 antibody (Cat. No. GP-K10, 1:500) on different human cell lines; secondary antibody: anti-guinea pig HRP (Cat. No. 90001, 1:5,000) Zoom

WB with anti-K10 antibody (Cat. No. GP-K10, 1:500) on different human cell lines; secondary antibody: anti-guinea pig HRP (Cat. No. 90001, 1:5,000)

anti-Keratin K10 guinea pig polyclonal + anti-guinea pig IgG HRP set

The set provides primary anti-Keratin K10 guinea pig polyclonal antibody and secondary anti-guinea pig IgG HRP antibody for western blot. 
The anti-Keratin K10 antibody is useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc.

Cat. No.: 71006
Quantity:  100 µl anti-Keratin K10 + 100 µg anti-guinea pig IgG HRP
No. of experiments:  ≤ 400 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume)

 

Cat. No. Product Name Reactivity Application
GP-K10 anti-Keratin K10 guinea pig polyclonal, serum human, mouse, rat ICC/IF, IHC, WB
90001 anti-guinea pig IgG goat polyclonal, HRP conjugate guinea pig ELISA, WB

Price as configured: $344.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Antibody Type polyclonal
Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Keratin K10 antibody: contains 0.09% sodium azide and 0.5% BSA; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
No. of experiments ≤ 400 (IHC, 50 µl/slide); ≤ 20 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin K10: 1:100 - 1:200
Immunohistochemistry (IHC) - paraffin anti-Keratin K10: 1:100 - 1:200 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K10: 1:250 – 1:1,000; anti-guinea pig HRP: 1:2,500 - 1:100,000

Background

Details

anti-Keratin K10 antibody:
MW 56,5000 (pI 5.3) intermediate filament polypeptide, keratin K10, detected by immunohistochemistry in the suprabasal layers of human epidermis (basal cells are negative). Useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc. Completely negative on non-stratified epithelia.
Positive control: Human suprabasal keratinocytes of epidermis; squamous cell carcinoma.

anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. GP-K10, anti-Keratin K10 guinea pig polyclonal, serum
Cat. No. 90001, anti-guinea pig IgG goat polyclonal, HRP conjugate

Reference

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