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WB with anti-K10 antibody (Cat. No. 11414, 1:200) on HEK293 whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500) Zoom

WB with anti-K10 antibody (Cat. No. 11414, 1:200) on HEK293 whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500)

anti-Keratin K10 mouse monoclonal, DE-K10 + anti-mouse IgG HRP set

The set provides primary anti-Keratin K10 mouse monoclonal antibody and secondary mouse IgG HRP antibody for western blot. 
The anti-Keratin K10 antibody specifically recognizes the 56.6 kD keratin K10 polypeptide in squamous cell carcinomas from e.g. epidermis, lung, bladder, cervix, esophagus.

Cat. No.: 71007
Quantity:  1 mL anti-Keratin K10 + 100 µg anti-mouse IgG HRP
No. of experiments:  ≤ 400 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume)

 

Cat. No. Product Name Reactivity Application
11414 anti-Keratin K10 mouse monoclonal, DE-K10, supernatant human IHC, WB
90002 anti-mouse IgG goat polyclonal, HRP conjugate mouse ELISA, WB

Price as configured: $309.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Keratin K10 antibody: contains 0.09% sodium azide; anti-mouse IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
No. of experiments ≤ 400 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin K10: 1:5 - 1:20
Immunohistochemistry (IHC) - paraffin anti-Keratin K10: 1:5 - 1:20 (protease treatment recommended)
Western Blot (WB) anti-Keratin K10: 1:100 – 1:250; anti-mouse HRP: 1:2,500 - 1:50,000

Background

Details

anti-Keratin K10 antibody:
MAb DE-K10 specifically recognizes the 56.6 kD keratin K10 (formerly also designated cytokeratin 10) polypeptide in squamous cells and carcinomas from e.g. epidermis, lung, bladder, cervix, oesophagus.
Positive control: Squamous cell carcinoma.

anti-mouse IgG goat polyclonal, HRP conjugate:
Whole mouse IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. 11414, anti-Keratin K10 mouse monoclonal, DE-K10, supernatant
Cat. No. 90002, anti-mouse IgG goat polyclonal, HRP conjugate

Reference

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