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IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin K18 mouse monoclonal, Ks18.04, liquid, purified, small

The anti-keratin K18 antibody decorates all simple epithelia and epithelium-derived neoplasms. For best performance, microwave treatment is recommended.
application_confirmed highly_published external_validation validated_by_epitope_mapping
Cat. No.: 690028S
Quantity:  0.2 mL (50 µg/mL)

Delivery Time: usually 1-7 working days

$170.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks18.04
Immunogen human keratin K18 from HeLa cytoskeletal preparation
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:20 -1:200
Immunohistochemistry (IHC) - paraffin 1:20 -1:200 (microwave treatment recommended)
Western Blot (WB) 1:100 - 1:500 (0.5 µg/ml - 0.1 µg/ml)

Background

Details

Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.

Tested cultured cell lines: MCF-7

Reference

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