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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | Ks18.04 + 18.27 + 18.5 + 9B1 + 18.174 |
| Immunogen | Keratin K18 of human and bovine origin |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 1000 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human, mammals (most mammals incl. mouse, rat, sheep, bovine, pig, dog, hamster) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:1 - 1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:1 - 1:10 (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
The antibody cocktail is excellent for discrimination of simple epithelia from those of different origin.
Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
Polypeptide reacting: Mr 45 kD (keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Reactivity on cultured cell lines: HeLa and MCF-7
Reference
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