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WB with anti-Keratin K19 antibody (Cat. No. 61010, 1:500) on MCF-7 whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500) Zoom

WB with anti-Keratin K19 antibody (Cat. No. 61010, 1:500) on MCF-7 whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500)

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6) + anti-mouse IgG HRP set

The set provides primary anti-Keratin K19 mouse monoclonal antibody and secondary mouse IgG HRP antibody for western blot. 
The anti-Keratin K19 antibody represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.

Cat. No.: 71013
Quantity:  50 µg anti-Keratin K19 + 100 µg anti-mouse IgG HRP

 

Cat. No. Product Name Reactivity Application
61029 anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), lyophilized, purified bovine, human, rabbit, rat ELISA, ICC/IF, IHC, WB
90002 anti-mouse IgG goat polyclonal, HRP conjugate mouse ELISA, WB

Price as configured: $302.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Keratin K19 antibody: lyophilized; reconstitute in 1 ml dist. water (0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4); anti-mouse IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin K19: 1:10 - 1:50
Immunohistochemistry (IHC) - paraffin anti-Keratin K19: 1:10 - 1:50 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K19: 1:50 - 1:500; anti-mouse HRP: 1:2,500 - 1:50,000

Background

Details

anti-Keratin K19 antibody:
Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma! Polypeptide Reacting: Mr 40 000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the α-helical fragment.
Tumors specifically detected: All tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma.
Reactivity on cultured cell lines: MCF-7.

anti-mouse IgG goat polyclonal, HRP conjugate:
Whole mouse IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. 61029, anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), lyophilized, purified
Cat. No. 90002, anti-mouse IgG goat polyclonal, HRP conjugate

Reference

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