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WB with anti-Keratin K20 antibody (Cat. No. 61026, 1:2,500) on RT-4 whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500) Zoom

WB with anti-Keratin K20 antibody (Cat. No. 61026, 1:2,500) on RT-4 whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500)

anti-Keratin K20 mouse monoclonal, IT-Ks20.8 + anti-mouse IgG HRP set

The set provides primary anti-Keratin K20 mouse monoclonal antibody and secondary mouse IgG HRP antibody for western blot. 
The anti-Keratin K20 antibody represents an excellent marker for gastrointestinal epithelium; specific for adenocarcinoma of the colon, transitional cell carcinoma of the bladder and Merkel cell tumors of the skin.

Cat. No.: 71014
Quantity:  50 µg anti-Keratin K20 + 100 µg anti-mouse IgG HRP

 

Cat. No. Product Name Reactivity Application
61026 anti-Keratin K20 mouse monoclonal, IT-Ks20.8, lyophilized, purified human, mouse IHC, WB
90002 anti-mouse IgG goat polyclonal, HRP conjugate mouse ELISA, WB

Price as configured: $411.00

Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Keratin K20 antibody: lyophilized; reconstitute in 1 ml dist. water (0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4); anti-mouse IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin K20: 1:10 - 1:100 (treatment with 0.02% Triton-X-100 recommended)
Immunohistochemistry (IHC) - paraffin anti-Keratin K20: 1:10 - 1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) anti-Keratin K20: 1:1,000 - 1:5,000; anti-mouse HRP: 1:2,500 - 1:50,000

Background

Details

anti-Keratin K20 antibody:
IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Polypeptide recognized: protein IT (keratin K20; Mr 46 000; formerly also designated cytokeratin 20).
Tested cultured cell lines: RT-4

.anti-mouse IgG goat polyclonal, HRP conjugate:
Whole mouse IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. 61026, anti-Keratin K20 mouse monoclonal, IT-Ks20.8, lyophilized, purified
Cat. No. 90002, anti-mouse IgG goat polyclonal, HRP conjugate

Reference

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