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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| 61431 |
anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22 |
FITC Conjugate | 250 µL |
$377.00
Excl. VAT, excl. Shipping Cost
|
| 61531 |
anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22 |
Biotin Conjugate | 250 µL |
$377.00
Excl. VAT, excl. Shipping Cost
|
| 65031 |
anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22 |
prediluted, purified | 5 mL |
$220.00
Excl. VAT, excl. Shipping Cost
|
| 61031 |
anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22 |
lyophilized, purified | 50 µg |
$289.00
Excl. VAT, excl. Shipping Cost
|
| 690031 |
anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22 |
liquid, purified | 1 mL (50 µg/mL) |
$425.00
Excl. VAT, excl. Shipping Cost
|
Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | Ks 5+8.22/C 22 |
| Immunogen | human keratin K8, purified from SDS PAGE gel |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4 |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | amphibia, bovine, human, hydra, mouse, pig |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10 - 1:100 |
| Immunohistochemistry (IHC) - paraffin | 1:10 - 1:100 (protease treatment and/or microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
C 22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia.
Polypeptide reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain of Keratin K8 (Waseem et al., 2004).
Reactivity on cultured cell lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK
Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004).
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