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WB with anti-Keratin K8 antibody (Cat. No. 61038, 1:100) on MCF-7 whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500) Zoom

WB with anti-Keratin K8 antibody (Cat. No. 61038, 1:100) on MCF-7 whole cell lysate; secondary antibody: anti-mouse HRP (Cat. No. 90002, 1:2,500)

anti-Keratin K8 mouse monoclonal, Ks8.7 + anti-mouse IgG HRP set

The set provides primary anti-Keratin K8 mouse monoclonal antibody and secondary mouse IgG HRP antibody for western blot. 
The anti-Keratin K8 antibody recognizes all simple-type epithelia and neoplasms derived therefrom. Stains all adenocarcinomas tested and undifferentiated carcinomas.

Cat. No.: 71010
Quantity:  50 µg anti-Keratin K8 + 100 µg anti-mouse IgG HRP

 

Cat. No. Product Name Reactivity Application
61038 anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified hamster, human ICC/IF, IHC, WB
90002 anti-mouse IgG goat polyclonal, HRP conjugate mouse ELISA, WB

Price as configured: $313.00

Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Keratin K8 antibody: lyophilized; reconstitute in 1 ml dist. water (0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4); anti-mouse IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin K8: 1:50 - 1:100
Immunohistochemistry (IHC) - paraffin anti-Keratin K8: 1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K8: 1:50 - 1:500; anti-mouse HRP: 1:2,500 - 1:50,000

Background

Details

anti-Keratin K8 antibody:
Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin.
Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia.
Reactivity on cultured cell lines: MCF-7
Tumors specifically detected: All adenocarcinoma tested; undifferentiated carcinoma; cervix carcinoma; hepatocellular carcinoma

anti-mouse IgG goat polyclonal, HRP conjugate:
Whole mouse IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. 61038, anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified
Cat. No. 90002, anti-mouse IgG goat polyclonal, HRP conjugate

Reference

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