Call +49 6221 8278-0

PROGEN - passion for research

close×

WB with anti-K8/K18 antibody (Cat. No. GP11, 1:2,000), MCF7 and PLC whole cell lysate (10 µg), secondary antibody: anti-guinea pig HRP (Cat. No. 90001, 1:50,000) Zoom

WB with anti-K8/K18 antibody (Cat. No. GP11, 1:2,000), MCF7 and PLC whole cell lysate (10 µg), secondary antibody: anti-guinea pig HRP (Cat. No. 90001, 1:50,000)

anti-Keratin K8/K18 guinea pig polyclonal + anti-guinea pig IgG HRP set

The set provides primary anti-Keratin K8/K18 guinea pig polyclonal antibody and secondary anti-guinea pig IgG HRP antibody for western blot. 
The anti-Keratin K8/K18 antibody is useful for research in simple and glandular epithelia and neoplasms derived therefrom.

Cat. No.: 71003
Quantity:  100 µl anti-Keratin K8/K18 + 100 µg anti-guinea pig IgG HRP

 

Cat. No. Product Name Reactivity Application
GP11 anti-Keratin K8/K18 guinea pig polyclonal, serum bovine, chicken, human, mouse, rat, Syrian hamster ELISA, IHC, WB
90001 anti-guinea pig IgG goat polyclonal, HRP conjugate guinea pig ELISA, WB

Price as configured: $366.00

Excl. Tax, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Specifications

Product description

Antibody Type polyclonal
Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Keratin K8/K18 antibody: contains 0.09% sodium azide; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin K8/K18: 1:100
Immunohistochemistry (IHC) - paraffin anti-Keratin K8/K18: 1:50 – 1:100 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K8/K18: 1:1,500 – 1:2,500; anti-guinea pig HRP: 1:2,500 - 1:100,000

Background

Details

anti-Keratin K8/K18 antibody:
Reactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma

anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. GP11, anti-Keratin K8/K18 guinea pig polyclonal, serum
Cat. No. 90001, anti-guinea pig IgG goat polyclonal, HRP conjugate

Reference

Reference

PublicationSpeciesApplication
Williams, J. et al. Epithelial and stromal circadian clocks are inversely regulated by their mechano-matrix environment. J.Cell.Sci. 131,  (2018). mouse ICC-IF
Linde, N. et al. Macrophages orchestrate breast cancer early dissemination and metastasis. Nat.Commun. 9, 21 (2018). mouse IHC (frozen)
Sarrach, S. et al. Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation. Sci.Rep. 8, 1801 (2018). mouse IHC (frozen)
Kakehashi, A. et al. Progression of Hepatic Adenoma to Carcinoma in Ogg1 Mutant Mice Induced by Phenobarbital. Oxid.Med.Cell.Longev. 2017, 8541064 (2017). mouse IHC (paraffin)
Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015). human IHC (paraffin)
Méndez-Gómez, H. R. et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol. Ther. Methods Clin. Dev. 2, (2015). mouse IHC (free-floating sections)
Song, Y. et al. Carcinoma Initiation via Rb Tumor Suppressor Inactivation: A Versatile Approach to Epithelial Subtype- Dependent Cancer Initiation in Diverse Tissues. PLoS One 8, (2013). mouse IHC (paraffin)
Dunphy, K. A. et al. Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int. 13, (2013). mouse IHC (paraffin), ICC-IF
König, K. et al. Loss of the Keratin Cytoskeleton Is Not Sufficient to Induce Epithelial Mesenchymal Transition in a Novel KRAS Driven Sporadic Lung Cancer Mouse Model. PLoS One 8, (2013). mouse IHC (paraffin)
Simmons, M. J., Serra, R., Hermance, N. & Kelliher, M. A. NOTCH1 inhibition in vivo results in mammary tumor regression and reduced mammary tumorsphere-forming activity in vitro. Breast Cancer Res. 14, R126 (2012). mouse WB, IHC (paraffin), ICC-IF
Clemons, N. J. et al. Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett’s esophagus. AJP Gastrointest. Liver Physiol. 303, G1335–G1346 (2012). mouse WB
Kumar, P. et al. Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8, (2012). mouse IHC (paraffin)
Yan, H. et al. Pathways Contributing to Development of Spontaneous Mammary Tumors in BALB/c-Trp53+/− Mice. Am. J. Pathol. 176, 1421–1432 (2010). mouse IHC (paraffin)
Bennecke, M. et al. Ink4a/Arf and Oncogene-Induced Senescence Prevent Tumor Progression during Alternative Colorectal Tumorigenesis. Cancer Cell 18, 135–146 (2010). mouse IHC (paraffin)
Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480–492 (2010). human IHC (frozen)
Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83–93 (2009). human IHC (frozen)
Li, Y.-C., Bai, W.-Z., Sakai, K. & Hashikawa, T. Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes. J. Histochem. Cytochem. Histochem Cytochem 57, 741–751 (2009). rat IHC (free-floating sections)
Hü Semann, Y. et al. Systemic Spread Is an Early Step in Breast Cancer. Cancer Cell 13, 58–68 (2008). mouse ICC-IF
Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936–1946 (2008). human, bovine IHC (frozen)
Bultman, S. J. et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene 27, 460–468 (2008). mouse IHC (paraffin)
Herschkowitz, J. I. et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 8, (2007). human IHC (paraffin)
Mikaelian, I. et al. Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development. Vet. Pathol. 43, 36–49 (2006). mouse WB, IHC (paraffin)
Moll, I. et al. Human Merkel cells - aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005). human IHC (frozen)
Galiè, M. et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis 26, 1868–1878 (2005). mouse IHC (paraffin)
Greten, F. R. et al. Stat3 and NF-kB Activation Prevents Apoptosis in Pancreatic Carcinogenesis. Gastroenterology 123, 2052–2063 (2002). mouse WB
Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67–79 (1989). human WB, ICC-IF
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987). human IHC (frozen), IEM

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields