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WB with anti-K8/K18 antibody (Cat. No. GP11, 1:2,000), MCF7 and PLC whole cell lysate (10 µg), secondary antibody: anti-guinea pig HRP (Cat. No. 90001, 1:50,000) Zoom

WB with anti-K8/K18 antibody (Cat. No. GP11, 1:2,000), MCF7 and PLC whole cell lysate (10 µg), secondary antibody: anti-guinea pig HRP (Cat. No. 90001, 1:50,000)

anti-Keratin K8/K18 guinea pig polyclonal + anti-guinea pig IgG HRP set

The set provides primary anti-Keratin K8/K18 guinea pig polyclonal antibody and secondary anti-guinea pig IgG HRP antibody for western blot. 
The anti-Keratin K8/K18 antibody is useful for research in simple and glandular epithelia and neoplasms derived therefrom.

Cat. No.: 71003
Quantity:  100 µl anti-Keratin K8/K18 + 100 µg anti-guinea pig IgG HRP
No. of experiments:  ≤ 200 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume)

 

Cat. No. Product Name Reactivity Application
GP11 anti-Keratin K8/K18 guinea pig polyclonal, serum bovine, chicken, human, mouse, rat, Syrian hamster ELISA, IHC, WB
90001 anti-guinea pig IgG goat polyclonal, HRP conjugate guinea pig ELISA, WB

Price as configured: $458.00

Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Antibody Type polyclonal
Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Keratin K8/K18 antibody: contains 0.09% sodium azide; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
No. of experiments ≤ 200 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin K8/K18: 1:100
Immunohistochemistry (IHC) - paraffin anti-Keratin K8/K18: 1:50 – 1:100 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K8/K18: 1:1,500 – 1:2,500; anti-guinea pig HRP: 1:2,500 - 1:100,000

Background

Details

anti-Keratin K8/K18 antibody:
Reactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma

anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. GP11, anti-Keratin K8/K18 guinea pig polyclonal, serum
Cat. No. 90001, anti-guinea pig IgG goat polyclonal, HRP conjugate

Reference

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