Call +49 6221 8278-0

PROGEN - passion for research

close×

WB with Keratin K8/K18 antibody (Cat. No. GP11, 1:2000), MCF7 whole cell lysate (Cat. No. 64004, 1.3 - 10 µg) Zoom

WB with Keratin K8/K18 antibody (Cat. No. GP11, 1:2000), MCF7 whole cell lysate (Cat. No. 64004, 1.3 - 10 µg)

anti-Keratin K8/K18 + positive western blot control

The set provides anti-Keratin K8/K18 antibody and associated western blot control - positively tested MCF7 cell lysate.
The set contains enough western blot control to perform 5 western blots.
The anti-Keratin K8/K18 antibody decorates simple and glandular epithelia and neoplasms derived therefrom.

Cat. No.: GP11-WBC
Quantity:  100 µl anti-Keratin K8/K18 + 50 µg western blot control
No. of experiments:  ≤ 200 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume)

 

Cat. No. Product Name Reactivity Application
GP11 anti-Keratin K8/K18 guinea pig polyclonal, serum bovine, chicken, human, mouse, rat, Syrian hamster ELISA, IHC, WB
64004 Positive western blot control: anti-Keratin K8/K18 antibody WB

Price as configured: $405.00

Excl. VAT, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18
Purification cell culture lysate, stabilized antiserum
Conjugate unconjugated
Formulation antibody: contains 0.09% sodium azide; western blot control: lyophilized, reconstitute in 50 µL 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)
Storage antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components
No. of experiments ≤ 200 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity bovine, chicken, human, mouse, rat, Syrian hamster

Applications

Tested applications Tested dilutions
ELISA anti-Keratin K8/K18: assay dependent
Immunohistochemistry (IHC) - frozen anti-Keratin K8/K18: 1:100
Immunohistochemistry (IHC) - paraffin anti-Keratin K8/K18: 1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K8/K18: 1:1,500 - 1:2,500; western blot control: 10 µg total protein per lane

Background

Details

anti-Keratin K8/K18 antibody:
eactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma

Positive western blot control:
Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.
MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.

Set content:
Cat. No. GP11, anti-Keratin K8/K18 guinea pig polyclonal, serum
Cat. No. 64004, Positive western blot control: anti-Keratin K8/K18 antibody

Reference

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields