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WB with anti-Pan antibody (Cat. No. GP14, 1:2,000), MCF7 whole cell lysate (1.3 - 10 µg), secondary antibody: anti-guinea pig HRP (Cat. No. 90001, 1:5,000) Zoom

WB with anti-Pan antibody (Cat. No. GP14, 1:2,000), MCF7 whole cell lysate (1.3 - 10 µg), secondary antibody: anti-guinea pig HRP (Cat. No. 90001, 1:5,000)

anti-Keratin Pan guinea pig polyclonal + anti-guinea pig IgG HRP set

The set provides primary anti-Keratin Pan guinea pig polyclonal antibody and secondary anti-guinea pig IgG HRP antibody for western blot. 
The anti-Keratin Pan antibody is decorates all types of epithelia and epithelium-derived neoplasms.

Cat. No.: 71004
Quantity:  100 µl anti-Keratin Pan + 100 µg anti-guinea pig IgG HRP
No. of experiments:  ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

 

Cat. No. Product Name Reactivity Application
GP14 anti-Keratin Pan guinea pig polyclonal, serum human, mouse ICC/IF, IHC, WB
90001 anti-guinea pig IgG goat polyclonal, HRP conjugate guinea pig ELISA, WB

Price as configured: $327.00

Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Antibody Type polyclonal
Immunogen see individual antibody datasheet for information about specific immunogens
Formulation Keratin Pan antibody: contains 0.09% sodium azide and 0.5% BSA; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Storage lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)
No. of experiments ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-Keratin Pan: 1:100 - 1:200
Immunohistochemistry (IHC) - paraffin anti-Keratin Pan: 1:50 – 1:200 (microwave treatment recommended)
Western Blot (WB) anti-Keratin Pan: 1:1,000 – 1:2,000; anti-guinea pig HRP: 1:2,500 - 1:100,000

Background

Details

anti-Keratin Pan antibody:
Reactive Polypeptides: Acidic (K1, K4, K8) and basic (K14) keratins (Mr 40,000 - Mr 68,000 polypeptides).
Tumors specifically detected: All types of epithelia and epithelium-derived neoplasms.

anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. GP14, anti-Keratin Pan guinea pig polyclonal, serum
Cat. No. 90001, anti-guinea pig IgG goat polyclonal, HRP conjugate

Reference

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