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Product description
| Antibody Type | polyclonal |
|---|---|
| Immunogen | see individual antibody datasheet for information about specific immunogens |
| Formulation | Keratin Pan antibody: contains 0.09% sodium azide and 0.5% BSA; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4) |
| Storage | lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles) |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | research use only |
| Tested species reactivity | see individual antibody datasheet |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | anti-Keratin Pan: 1:100 - 1:200 |
| Immunohistochemistry (IHC) - paraffin | anti-Keratin Pan: 1:50 – 1:200 (microwave treatment recommended) |
| Western Blot (WB) | anti-Keratin Pan: 1:1,000 – 1:2,000; anti-guinea pig HRP: 1:2,500 - 1:100,000 |
Background
Details
anti-Keratin Pan antibody:
Reactive Polypeptides: Acidic (K1, K4, K8) and basic (K14) keratins (Mr 40,000 - Mr 68,000 polypeptides).
Tumors specifically detected: All types of epithelia and epithelium-derived neoplasms.
anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Set content:
Cat. No. GP14, anti-Keratin Pan guinea pig polyclonal, serum
Cat. No. 90001, anti-guinea pig IgG goat polyclonal, HRP conjugate
Reference
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Species
Application
human
IHC (paraffin)
human
IHC (paraffin)
human
IHC (paraffin)
