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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | X67 |
| Immunogen | Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells) |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | bovine, human, mouse, rat, rat kangoroo, trout (negativ with rat and rat kangaroo) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:10 |
| Immunohistochemistry (IHC) - frozen | 1:10 |
| Western Blot (WB) | assay dependent |
Background
Details
The monoclonal antibody cocktail decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus.
Polypeptides reacting: Lamin isotypes of Mr 60 - 75 kD
Antigen recognized in species:
| Xenopus laevis | LA, LI, LII |
| bovine | LA, C |
| mouse | n.d. |
| rat | - |
| human | LA, C |
| trout | LA, LI, LII |
| rat kangaroo | - |
Reference
There are currently no references for this product.
