ELISA with B62-FE2 HRP conjugate (Cat. No. 61097-HRP) as detection antibody (1:1000; 10 ng antibody per well)(courtesy of P. Heine, TU Braunschweig)

anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2, HRP Conjugate, sample

The antibody binds to pVIII major coat protein of filamentous phages of the fd, F1 and M13 group. Suitable for the identification of recombinant phages in antigen and antibody-phage display systems and phage ELISA.

Cat. No.: 61097-HRPS
Quantity: 250 µL (100 µg/mL)

Delivery Time: usually 1-7 working days

$119.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Rondot, S., Koch, J., Breitling, F. & DÃ¼bel, S. A helper phage to improve single-chain antibody presentation in phage display. Nat. Biotechnol. 19, 75-8 (2001).

M13

ELISA

Kneissel, S. et al. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-8 (1999).

Micheel, B. et al. Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages. J. Immunol. Methods 171, 103-9 (1994).

IEM
ELISA

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Specifications

Datasheet

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
61097	anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2	lyophilized, purified, 100 µg	100 µg	$227.00 Excl. VAT, excl. Shipping Cost
61597	anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2	Biotin Conjugate	250 µL (100 µg/mL)	$351.00 Excl. VAT, excl. Shipping Cost
61497	anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2	FITC Conjugate	250 µL (400 µg/mL)	$531.00 Excl. VAT, excl. Shipping Cost
61997	anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2	lyophilized, purified, sample	50 µg	$119.00 Excl. VAT, excl. Shipping Cost
61097-HRP	anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2	HRP Conjugate	1 mL (100 µg/mL)	$319.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG2b
Clone	B62-FE2
Immunogen	fd phages from E. coli F+ strain (JM109)
Purification	protein A affinity chromatography
Conjugate	peroxidase/ HRP
Formulation	HRP stabilizer (Candor 222902)
Storage	store at 2-8°C
Intended use	Research use only
Tested species reactivity	filamentous phages F1, filamentous phages fd, filamentous phages M13

Applications

Tested applications	Tested dilutions
ELISA	1:250 - 1:1,000 (0.1 - 0.4 µg/mL)
Phage Display	immunoassays for the identification of recombinant antigen- or antibody-phages; Detection limit: 10^7 phage particles

Background

Details

B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (Kneissel et al., 1999).

Kneissel, S. et al. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21–8 (1999).

Reference