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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | P32 (26S-161) |
| Immunogen | 26S complexes of Xenopus laevis oocytes |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 1000 (WB, 5 ml reaction volume) |
| Intended use | Research use only |
| Tested species reactivity | human, mouse, rat, rat kangoroo |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | ready-to-use |
| Western Blot (WB) | 1:1,000 |
Background
Details
Recognizes the 20S subcomplex within the 26S hetero-oligomeric protein complex and the free cytosolic form of 20S cylinder par¬ticles; the epitope is resistant to formaldehyde fixation (up to 4%). P32 (26S-161) is not suitable for immunoprecipitation.
Polypeptide reacting: p32 subunit of 20S cylinder particles.
Reactivity in cultured cell lines: XLKE-A6 (Xenopus), PLC, MCF-7, A431, CaCo (human); 3T3 (mouse); RV (rat); Ptk2 (rat kangaroo).
Reference
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