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Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | P52 (26S-17) (17.14.1) |
Immunogen | 26S proteasomes purified from Xenopus laevis ovary |
Purification | hybridoma cell culture supernatant |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 10 (WB, 5 ml reaction volume) |
Intended use | Research use only |
Tested species reactivity | human, rat, rat kangoroo (negative with mouse) |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | not recommended |
Immunoprecipitation (IP) | not recommended |
Western Blot (WB) | 1:5 - 1:10 |
Background
Details
The p52 subunit (Mr 52.000) is the major antigen of the 19S cap complex, which is a subcomplex of the 26S proteasome, implicated in ATP- and ubiquitin-dependent protein degradation.