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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | P52 (26S-17) (17.14.1) |
| Immunogen | 26S proteasomes purified from Xenopus laevis ovary |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 10 (WB, 5 ml reaction volume) |
| Intended use | Research use only |
| Tested species reactivity | human, rat, rat kangoroo (negative with mouse) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | not recommended |
| Immunoprecipitation (IP) | not recommended |
| Western Blot (WB) | 1:5 - 1:10 |
Background
Details
The p52 subunit (Mr 52.000) is the major antigen of the 19S cap complex, which is a subcomplex of the 26S proteasome, implicated in ATP- and ubiquitin-dependent protein degradation.
