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anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, RCK102, supernatant

Reacts with keratins K5/K8 present in all epithelial cell types; no reaction in non-epithelial tissues. Useful for the discrimination of carcinomas from non-epithelial tumors.
Cat. No.: 10521
Quantity:  1 mL

Delivery Time: usually 1-7 working days

€228.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone RCK102
Immunogen lung cancer cell preparation
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity dog, hamster, human, mouse, pig, rabbit, rat (most mammals)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:5 - 1:10
Immunohistochemistry (IHC) - paraffin 1:5 - 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Mab RCK 102, directed against the 58 kD and 52.2 kD keratin proteins (keratins K5 and K8; formerly also designated cytokeratins 5 and 8 in the Moll catalogue of keratins), reacts with all epithelial cell types. No reaction is observed in non-epithelial tissues. The antibody can be used for the discrimination of carcinomas from non-epithelial tumors such as lymphomas, sarcomas and neural tumors. RCK 102 is applicable on frozen and paraffin-embedded tissue. Formalin fixation may cause antigen denaturation or masking. Therefore, protease digestion is necessary prior to antibody application.

Positive control: Skin, cervix.

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