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WB with anti-Keratin pan antibody (Cat. No. GP14, 1:2000), MCF7 whole cell lysate (Cat. No. 64005, 1.3 - 10 µg) Zoom

WB with anti-Keratin pan antibody (Cat. No. GP14, 1:2000), MCF7 whole cell lysate (Cat. No. 64005, 1.3 - 10 µg)

anti-Keratin pan + positive western blot control

The set provides anti-Keratin pan antibody and associated western blot control - positively tested MCF7 cell lysate.
The set contains enough western blot control to perform 5 western blots.
The anti-Keratin pan antibody decorates all types of epithelia and epithelium-derived neoplasms.

Cat. No.: GP14-WBC
Quantity:  100 µl anti-Keratin pan + 50 µg western blot control

 

Cat. No. Product Name Reactivity Application
GP14 anti-Keratin Pan guinea pig polyclonal, serum human, mouse ICC/IF, IHC, WB
64005 Positive western blot control: anti-Keratin pan antibody WB

Price as configured: €285.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptides of human keratins
Purification cell culture lysate, stabilized antiserum
Conjugate unconjugated
Formulation antibody: contains 0.09% sodium azide and 0.5% BSA; western blot control: lyophilized, reconstitute in 50 µL 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)
Storage antibody: short term at 2 – 8 °C; long term in aliquots at - 20 °C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) anti-Keratin pan: assay dependent
Immunohistochemistry (IHC) - frozen anti-Keratin pan: 1:100 - 1:200
Immunohistochemistry (IHC) - paraffin anti-Keratin pan: 1:50 - 1:200 (microwave treatment recommended)
Western Blot (WB) anti-Keratin pan: 1:1,000 - 1:2,000; western blot control: 10 µg total protein per lane

Background

Details

anti-Keratin pan antibody:
Reactive Polypeptides: Acidic (K1, K4, K8) and basic (K14) keratins (Mr 40,000 - Mr 68,000 polypeptides).
Tumors specifically detected: All types of epithelia and epithelium-derived neoplasms.

Positive western blot control:
Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.
MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.

Set content:
Cat. No. GP14, anti-Keratin Pan guinea pig polyclonal, serum
Cat. No. 64005, Positive western blot control: anti-Keratin pan antibody

Reference

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