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IHC of human kidney using anti-Keratin K18 antibody (Cat. No. 61028, 1:20) (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human kidney using anti-Keratin K18 antibody (Cat. No. 61028, 1:20) (courtesy of J.Heß, University Hospital Heidelberg)

anti-Keratin sample set

The anti-Keratin sample set provides antibodies directed against Keratin K17, K18, K19, K8/18 and pan to evaluate the presence and status in IHC and WB.
The set contains enough antibody to perform stainings on 6-12 sections per antibody.

Cat. No.: 70020
Quantity:  600 µl each antibody

 

Cat. No. Product Name Reactivity Application
61036S anti-Keratin K17 mouse monoclonal, Ks17.E3, purified, sample (ready-to-use) human, rat IHC, WB
61028S anti-Keratin K18 mouse monoclonal, Ks18.04, purified, sample (ready-to-use) bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish ICC/IF, IHC, WB
GP11S anti-Keratin K8/K18 guinea pig polyclonal, serum, sample (ready-to-use) bovine, chicken, human, mouse, rat, Syrian hamster ELISA, IHC, WB
61029S anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), purified, sample (ready-to-use) bovine, human, rabbit, rat ELISA, ICC/IF, IHC, WB
GP14S anti-Keratin Pan guinea pig polyclonal, serum, sample (ready-to-use) human, mouse ICC/IF, IHC, WB

Price as configured: €184.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Number of experiments 6-12 sections per antibody
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use
Western Blot (WB) assay dependent

Background

Details

Keratins are a large protein family. Expression and structure of its members is highly tissue and differentiation specific. As heteropolymers, keratins form intermediate filaments (IFs) with the primary task of providing a structural framework in epithelial cells that protects them from mechanical and non-mechanical stress. In addition, keratins are involved in establishing apico-basal polarization, regulating motility and cell size and even play a role in complex cellular events such as protein synthesis, membrane traffic or cell signaling.
Keratins are popular diagnostic markers in cancer because of their typical signature for tumor cell type and differentiation, while maintaining the specific expression pattern associated with the cell type of origin. Commonly used markers in the immunohistochemical analysis of tumors are K5-K8 and K18-K20. Adenocarcinomas (epithelial cancers arising in glandular tissues) build up the largest group of human epithelial malignancies. As they can originate in various organs, the ability of differentiating a carcinoma according to the tissue of origin is crucial. By using epithelial keratins as diagnostic markers, the best treatment depending on the exact type of cancer can be determined. In general, most adenocarcinomas express K8, K18 and K19. K7 and K20 levels vary depending on cancer type. In unclear cases, keratin typing is often key to assess the correct tumor type. Beyond their role as diagnostic markers, keratins are also useful prognostic indicators in epithelial malignancies.

The anti-Keratin sample set provides antibodies directed against Keratin K17, K18, K19, K8/18 and pan to evaluate the presence and status in IHC and WB.
The set contains enough antibody to perform stainings on 6-12 sections per antibody.

Set content:
Cat. No. 61036S, anti-Keratin K17 mouse monoclonal, Ks17.E3, purified, sample, 600 µl
Cat. No. 61028S, anti-Keratin K18 mouse monoclonal, Ks18.04, purified, sample, 600 µl
Cat. No. GP11S, anti-Keratin K8/K18 guinea pig polyclonal, serum, sample, 600 µl
Cat. No. 61029S, anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), purified, sample, 600 µl
Cat. No. GP14S, anti-Keratin Pan guinea pig polyclonal, serum, sample, 600 µl

Reference

Reference

PublicationSpeciesApplicationAntibody
Sawitza, I., Kordes, C., Götze, S., Herebian, D. & Häussinger, D. Bile acids induce hepatic differentiation of mesenchymal stem cells. Sci. Rep. 5, (2015). rat ICC-IF 61029
Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015). human IHC (paraffin) GP11
Soady, K. J. et al. Mouse mammary stem cells express prognostic markers for triple-negative breast cancer. Breast Cancer Res. 17, (2015). mouse ICC-IF 61028
Lokody, I. B., Francis, J. C., Gardiner, J. R., Erler, J. T. & Swain, A. Pten Regulates Epithelial Cytodifferentiation during Prostate Development. PLoS One 10, (2015). mouse IHC (paraffin) 61028
Méndez-Gómez, H. R. et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol. Ther. Methods Clin. Dev. 2, (2015). mouse IHC (free-floating sections) GP11
Zatloukal, B. et al. Sensitivity and Specificity of In situ Proximity Ligation for Protein Interaction Analysis in a Model of Steatohepatitis with Mallory-Denk Bodies. PLoS One 9, (2014). mouse IHC (frozen) 61028
Eberle, J. et al. Receptors for short-chain fatty acids in brush cells at the "gastric groove". Front. Physiol. 5, (2014). mouse IHC (frozen) 61028
Foth, M. et al. Fibroblast Growth Factor Receptor 3 activation plays a causative role in urothelial cancer pathogenesis in cooperation with Pten loss in mice. J. Pathol. 233, 148–158 (2014). mouse IHC (paraffin) 61028
Eberle, J. et al. Band-like arrangement of taste-like sensory cells at the gastric groove: evidence for paracrine communication. Front. Physiol. 4, (2013). mouse IHC (frozen) 61028
Eberle, J. et al. Putative interaction of brush cells with bicarbonate secreting cells in the proximal corpus mucosa. Front. Physiol. 4, (2013). mouse IHC (frozen) 61028
Song, Y. et al. Carcinoma Initiation via Rb Tumor Suppressor Inactivation: A Versatile Approach to Epithelial Subtype- Dependent Cancer Initiation in Diverse Tissues. PLoS One 8, (2013). mouse IHC (paraffin) GP11
Dunphy, K. A. et al. Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int. 13, (2013). mouse IHC (paraffin), ICC-IF GP11
König, K. et al. Loss of the Keratin Cytoskeleton Is Not Sufficient to Induce Epithelial Mesenchymal Transition in a Novel KRAS Driven Sporadic Lung Cancer Mouse Model. PLoS One 8, (2013). mouse IHC (paraffin) GP11
Simmons, M. J., Serra, R., Hermance, N. & Kelliher, M. A. NOTCH1 inhibition in vivo results in mammary tumor regression and reduced mammary tumorsphere-forming activity in vitro. Breast Cancer Res. 14, R126 (2012). mouse WB, IHC (paraffin), ICC-IF GP11
Clemons, N. J. et al. Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett’s esophagus. AJP Gastrointest. Liver Physiol. 303, G1335–G1346 (2012). mouse WB GP11
Kumar, P. et al. Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8, (2012). mouse IHC (paraffin) GP11
Barbone, D. et al. Vorinostat Eliminates Multicellular Resistance of Mesothelioma 3D Spheroids via Restoration of Noxa Expression. PLoS One 7, (2012). human IHC (paraffin) GP14
Barbone, D. et al. The Bcl-2 repertoire of mesothelioma spheroids underlies acquired apoptotic multicellular resistance. Cell Death Dis. 2, (2011). human IHC (paraffin) GP14
Yan, H. et al. Pathways Contributing to Development of Spontaneous Mammary Tumors in BALB/c-Trp53+/− Mice. Am. J. Pathol. 176, 1421–1432 (2010). mouse IHC (paraffin) GP11
Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55–73 (2010). human IHC (frozen) 61036
Bennecke, M. et al. Ink4a/Arf and Oncogene-Induced Senescence Prevent Tumor Progression during Alternative Colorectal Tumorigenesis. Cancer Cell 18, 135–146 (2010). mouse IHC (paraffin) GP11
Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480–492 (2010). human IHC (frozen) GP11
Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83–93 (2009). human IHC (frozen) GP11
Teissedre, B. et al. MMTV-Wnt1 and -DN89b-Catenin Induce Canonical Signaling in Distinct Progenitors and Differentially Activate Hedgehog Signaling within Mammary Tumors. PLoS One 4, (2009). mouse IHC (paraffin) 61028
Li, Y.-C., Bai, W.-Z., Sakai, K. & Hashikawa, T. Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes. J. Histochem. Cytochem. Histochem Cytochem 57, 741–751 (2009). rat IHC (free-floating sections) GP11
Campard, D., Lysy, P. A., Najimi, M. & Sokal, E. M. Native Umbilical Cord Matrix Stem Cells Express Hepatic Markers and Differentiate Into Hepatocyte-like Cells. Gastroenterology 134, (2008). human ICC-IF 61028
Hü Semann, Y. et al. Systemic Spread Is an Early Step in Breast Cancer. Cancer Cell 13, 58–68 (2008). mouse ICC-IF GP11
Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936–1946 (2008). human, bovine IHC (frozen) GP11
Bultman, S. J. et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene 27, 460–468 (2008). mouse IHC (paraffin) GP11
Herschkowitz, J. I. et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 8, (2007). human IHC (paraffin) GP11
Boehnke, K. et al. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur. J. Cell Biol. 86, 731–46 (2007). human IHC (paraffin) GP14
Nevado, C., Valverde, A. M. & Benito, M. Role of Insulin Receptor in the Regulation of Glucose Uptake in Neonatal Hepatocytes. Endocrinology 147, 3709–3718 (2006). mouse ICC-IF 61028
Mikaelian, I. et al. Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development. Vet. Pathol. 43, 36–49 (2006). mouse WB, IHC (paraffin) GP11
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen) 61036, 61028, 61029
Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005). human IHC (paraffin) 61036, 61028, GP11
Galiè, M. et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis 26, 1868–1878 (2005). mouse IHC (paraffin) GP11
Obermueller, E., Vosseler, S., Fusenig, N. E. & Mueller, M. M. Cooperative Autocrine and Paracrine Functions of Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor in the Progression of Skin Carcinoma Cells. CANCER Res. 64, 7801–7812 (2004). human IHC (frozen) GP14
Ikenouchi, J., Matsuda, M., Furuse, M. & Tsukita, S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J. Cell Sci. 116, 1959–1967 (2003). mouse WB, ICC-IF 61028
Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255–263 (2002). human ICC-IF 61028
Stumptner, C. Mallory body—A disease-associated type of sequestosome. Hepatology 35, 1053–1062 (2002). human, mouse IHC (frozen), IEM 61028
Welm, B. E. et al. Sca-1pos Cells in the Mouse Mammary Gland Represent an Enriched Progenitor Cell Population. Dev. Biol. 245, 42–56 (2002). mouse IHC (paraffin) 61028
Greten, F. R. et al. Stat3 and NF-kB Activation Prevents Apoptosis in Pancreatic Carcinogenesis. Gastroenterology 123, 2052–2063 (2002). mouse WB GP11
Bantel, H., Ruck, P., Gregor, M. & Schulze-Osthoff, K. Detection of elevated caspase activation and early apoptosis in liver diseases. Eur. J. Cell Biol. 80, 230–239 (2001). human WB, IHC (paraffin), ICC 61028
Romih, R., Jezernik, K. & Masera, A. Uroplakins and cytokeratins in the regenerating rat urothelium after sodium saccharin treatment. Histochem. Cell Biol. 109, 263–9 (1998). rat IHC (paraffin) 61036
Höchtlen-Vollmar, W. et al. Occult epithelial tumor cells detected in bone marrow by an enzyme immunoassay specific for cytokeratin 19. Int. J. cancer 70, 396–400 (1997). human ELISA 61029
Dittadi, R. et al. Standardization of assay for cytokeratin-related tumor marker CYFRA21.1 in urine samples. Clin. Chem. 42, 1634–8 (1996). human ELISA 61029
Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518–35 (1995). human IHC (paraffin) 61036
Petersen, G., Song, D., Hügle-Dörr, B., Oldenburg, I. & Bautz, E. K. Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries. Mol. Gen. Genet. MGG 249, 425–31 (1995). epitope mapping 61029
Möbus, V. J. et al. Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis. Br. J. Cancer 69, 422–428 (1994). human ICC-IF 61036, 61029
Van Der Gaast ’, A. et al. Evaluation of a new tumour marker in patients with non-small-cell lung cancer: Cyfra 21.1. Br. J. Cancer 69, 525–528 (1994). human ELISA 61029
Sugama, Y. et al. Clinical usefulness of CYFRA assay in diagnosing lung cancer: measurement of serum cytokeratin fragment. Jpn. J. Cancer Res. 85, 1178–84 (1994). human ELISA 61029
Grenier, J. et al. Cyfra 21-1, a new marker of lung cancer. Nucl. Med. Biol. 21, 471–476 (1994). human ELISA 61029
Moll, R. et al. The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation. 53, 75–93 (1993). human IHC (frozen) 61036, 61029
Pujol, J. L. et al. Serum fragment of cytokeratin subunit 19 measured by CYFRA 21-1 immunoradiometric assay as a marker of lung cancer. Cancer Res. 53, 61–6 (1993). human ELISA 61029
Stieber, P. et al. CYFRA 21-1. A new marker in lung cancer. Cancer 72, 707–13 (1993). human ELISA 61029
Smedts, F. et al. Keratin Expression in Cervical Cancer. Am. J. Pathol. 141, 497–511 (1992). human IHC (frozen) 61036
Smedts, F. et al. Basal-cell Keratins in Cervical Reserve Cells and a Comparison to Their Expression in Cervical Intraepithelial Neoplasia. Am. J. Pathol. 140, (1992). human WB, IHC (frozen) 61036
Wetzels, R. H. et al. Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18. Histopathology 20, 295–303 (1992). human IHC (frozen) 61036
Wetzels, R. H. W. et al. Basal Cell-specific and Hyperproliferation-related Keratins in Human Breast Cancer. Am. J. Pathol. 138, 751–763 (1991). human IHC (frozen) 61036

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