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IHC on human skin using anti-Keratin K10 antibody (Cat. No. 11414)(courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC on human skin using anti-Keratin K10 antibody (Cat. No. 11414)(courtesy of J.Heß, University Hospital Heidelberg)

anti-skin layer Keratin sample set

The anti-skin layer Keratin sample set provides antibodies directed against Keratin pan, K2e, K10, K14, K8/18 to evaluate the presence and status in IHC and WB.
The set contains enough antibody to perform stainings on 6-12 sections per antibody.

highly_published external_validation
Cat. No.: 70021
Quantity:  600 µl each antibody

 

Cat. No. Product Name Reactivity Application
GP14S anti-Keratin Pan guinea pig polyclonal, serum, sample (ready-to-use) human, mouse ICC/IF, IHC, WB
65177S anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant, sample (ready-to-use) human IHC, WB
11414S anti-Keratin K10 mouse monoclonal, DE-K10, supernatant, sample (ready-to-use) human IHC, WB
GP-CK14S anti-Keratin K14 guinea pig polyclonal, serum, sample (ready-to-use) human, mouse IHC, WB
GP11S anti-Keratin K8/K18 guinea pig polyclonal, serum, sample (ready-to-use) bovine, chicken, human, mouse, rat, Syrian hamster ELISA, IHC, WB

Price as configured: €184.00

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Number of experiments 6-12 sections per antibody
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use
Western Blot (WB) assay dependent

Background

Details

Keratins are a large protein family. Expression and structure of its members is highly tissue and differentiation specific. As heteropolymers, keratins form intermediate filaments (IFs) with the primary task of providing a structural framework in epithelial cells that protects them from mechanical and non-mechanical stress. In addition, keratins are involved in establishing apico-basal polarization, regulating motility and cell size and even play a role in complex cellular events such as protein synthesis, membrane traffic or cell signaling.
Keratins are popular diagnostic markers in cancer because of their typical signature for tumor cell type and differentiation, while maintaining the specific expression pattern associated with the cell type of origin. Commonly used markers in the immunohistochemical analysis of tumors are K5-K8 and K18-K20. Adenocarcinomas (epithelial cancers arising in glandular tissues) build up the largest group of human epithelial malignancies. As they can originate in various organs, the ability of differentiating a carcinoma according to the tissue of origin is crucial. By using epithelial keratins as diagnostic markers, the best treatment depending on the exact type of cancer can be determined. In general, most adenocarcinomas express K8, K18 and K19. K7 and K20 levels vary depending on cancer type. In unclear cases, keratin typing is often key to assess the correct tumor type. Beyond their role as diagnostic markers, keratins are also useful prognostic indicators in epithelial malignancies.

The anti-skin layer Keratin sample set provides antibodies directed against Keratin pan, K2e, K10, K14, K8/18 to evaluate the presence and status in IHC and WB.
The set contains enough antibody to perform stainings on 6-12 sections per antibody.

Set content:
Cat. No. GP14S, anti-Keratin Pan guinea pig polyclonal, serum, sample, 600 µl
Cat. No. 65177S, anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant, sample, 600 µl
Cat. No. 11414S, anti-Keratin K10 mouse monoclonal, DE-K10, supernatant, sample, 600 µl
Cat. No. GP-CK14S, anti-Keratin K14 guinea pig polyclonal, serum, sample, 600 µl
Cat. No. GP11S, anti-Keratin K8/K18 guinea pig polyclonal, serum, sample, 600 µl

Reference

Reference

PublicationSpeciesApplicationAntibody
Yosef, R. et al. Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL. Nat. Commun. 7, (2016). mouse IHC (paraffin) GP-CK14
Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015). human WB, IHC (paraffin) GP-CK14, GP11
Méndez-Gómez, H. R. et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol. Ther. Methods Clin. Dev. 2, (2015). mouse IHC (free-floating sections) GP11
Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014). mouse IHC (frozen) 11414, GP-CK14
Cui, C.-Y. et al. Involvement of Wnt, Eda and Shh at defined stages of sweat gland development. Development 141, 3752–60 (2014). mouse IHC (paraffin) GP-CK14
Tokarsky-Amiel, R. et al. Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis. Cancer Res. 73, 2829–2839 (2013). mouse IHC (paraffin) GP-CK14
Song, Y. et al. Carcinoma Initiation via Rb Tumor Suppressor Inactivation: A Versatile Approach to Epithelial Subtype- Dependent Cancer Initiation in Diverse Tissues. PLoS One 8, (2013). mouse IHC (paraffin) GP11
Langbein, L. et al. New facets of keratin K77: Interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793–812 (2013). human, mouse IHC (frozen) 11414
Dunphy, K. A. et al. Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int. 13, (2013). mouse IHC (paraffin), ICC-IF GP11
König, K. et al. Loss of the Keratin Cytoskeleton Is Not Sufficient to Induce Epithelial Mesenchymal Transition in a Novel KRAS Driven Sporadic Lung Cancer Mouse Model. PLoS One 8, (2013). mouse IHC (paraffin) GP11
Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750–1758 (2012). mouse IHC (frozen) 11414, GP-CK14
Barbone, D. et al. Vorinostat Eliminates Multicellular Resistance of Mesothelioma 3D Spheroids via Restoration of Noxa Expression. PLoS One 7, (2012). human IHC (paraffin) GP14
Kumar, P. et al. Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8, (2012). mouse mouse GP11
Simmons, M. J., Serra, R., Hermance, N. & Kelliher, M. A. NOTCH1 inhibition in vivo results in mammary tumor regression and reduced mammary tumorsphere-forming activity in vitro. Breast Cancer Res. 14, R126 (2012). mouse WB, IHC (paraffin), ICC-IF GP11
Clemons, N. J. et al. Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett’s esophagus. AJP Gastrointest. Liver Physiol. 303, G1335–G1346 (2012). mouse WB GP11
Cui, C.-Y. et al. Forkhead transcription factor FoxA1 regulates sweat secretion through Bestrophin 2 anion channel and Na-K-Cl cotransporter 1. Proc. Natl. Acad. Sci. United States Am.  109, 1199–1203 (2012). mouse IHC (paraffin) GP-CK14
Barbone, D. et al. The Bcl-2 repertoire of mesothelioma spheroids underlies acquired apoptotic multicellular resistance. Cell Death Dis. 2, (2011). human IHC (paraffin) GP14
Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55–73 (2010). human IHC (frozen) GP-CK14
Yan, H. et al. Pathways Contributing to Development of Spontaneous Mammary Tumors in BALB/c-Trp53+/− Mice. Am. J. Pathol. 176, 1421–1432 (2010). mouse IHC (paraffin) GP11
Bennecke, M. et al. Ink4a/Arf and Oncogene-Induced Senescence Prevent Tumor Progression during Alternative Colorectal Tumorigenesis. Cancer Cell 18, 135–146 (2010). mouse IHC (paraffin) GP11
Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480–492 (2010). human IHC (frozen) GP11
Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83–93 (2009). human IHC (frozen) GP11
Li, Y.-C., Bai, W.-Z., Sakai, K. & Hashikawa, T. Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes. J. Histochem. Cytochem. Histochem Cytochem 57, 741–751 (2009). rat IHC (free-floating sections) GP11
Hü Semann, Y. et al. Systemic Spread Is an Early Step in Breast Cancer. Cancer Cell 13, 58–68 (2008). mouse ICC-IF GP11
Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936–1946 (2008). human, bovine IHC (frozen) GP11
Bultman, S. J. et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene 27, 460–468 (2008). mouse IHC (paraffin) GP11
Boehnke, K. et al. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur. J. Cell Biol. 86, 731–46 (2007). human IHC (paraffin) 11414
Herschkowitz, J. I. et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 8, (2007). human IHC (paraffin) GP11
Mikaelian, I. et al. Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development. Vet. Pathol. 43, 36–49 (2006). mouse WB, IHC (paraffin) GP11
Jennemann, R. et al. Integrity and Barrier Function of the Epidermis Critically Depend on Glucosylceramide Synthesis. J. Biol. Chem. 282, 3083–3094 (2006). mouse IHC (paraffin) GP-CK14
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen) 11414,GP-CK14
Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761–774 (2005). human IHC (frozen) GP-CK14
Moll, I. et al. Human Merkel cells - aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005). human IHC (frozen) GP11
Galiè, M. et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis 26, 1868–1878 (2005). mouse IHC (paraffin) GP11
Obermueller, E., Vosseler, S., Fusenig, N. E. & Mueller, M. M. Cooperative Autocrine and Paracrine Functions of Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor in the Progression of Skin Carcinoma Cells. CANCER Res. 64, 7801–7812 (2004). human IHC (frozen) GP14
Langbein, L. et al. Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur. J. Cell Biol. 82, 385–400 (2003). human, bovine IHC (frozen) GP-CK14
Herrmann, T. et al. Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. J. Cell Biol. 161, 1105–1115 (2003). mouse IHC (paraffin) GP-CK14
Greten, F. R. et al. Stat3 and NF-kB Activation Prevents Apoptosis in Pancreatic Carcinogenesis. Gastroenterology 123, 2052–2063 (2002). mouse WB GP11
Corver, W. E. et al. Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer. Cytometry 39, 96–107 (2000). human FACS 11414
Moll, I., Houdek, P., Schäfer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437–46 (1999). human IHC (paraffin) 11414
Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67–79 (1989). human WB, ICC-IF GP11
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987). human IHC (frozen), IEM GP11

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