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IHC on human skin using anti-Keratin K10 antibody (Cat. No. 11414)(courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC on human skin using anti-Keratin K10 antibody (Cat. No. 11414)(courtesy of J.Heß, University Hospital Heidelberg)

anti-skin layer Keratin sample set

The anti-skin layer Keratin sample set provides antibodies directed against Keratin pan, K2e, K10, K14, K8/18 to evaluate the presence and status in IHC and WB.
The set contains enough antibody to perform stainings on 6-12 sections per antibody.

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Cat. No.: 70021
Quantity:  600 µl each antibody

 

Cat. No. Product Name Reactivity Application
GP14S anti-Keratin Pan guinea pig polyclonal, serum, sample (ready-to-use) human, mouse ICC/IF, IHC, WB
65177S anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant, sample (ready-to-use) human IHC, WB
11414S anti-Keratin K10 mouse monoclonal, DE-K10, supernatant, sample (ready-to-use) human IHC, WB
GP-CK14S anti-Keratin K14 guinea pig polyclonal, serum, sample (ready-to-use) human, mouse IHC, WB
GP11S anti-Keratin K8/K18 guinea pig polyclonal, serum, sample (ready-to-use) bovine, chicken, human, mouse, rat, Syrian hamster ELISA, IHC, WB

Price as configured: $230.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Number of experiments 6-12 sections per antibody
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use
Western Blot (WB) assay dependent

Background

Details

Keratins are a large protein family. Expression and structure of its members is highly tissue and differentiation specific. As heteropolymers, keratins form intermediate filaments (IFs) with the primary task of providing a structural framework in epithelial cells that protects them from mechanical and non-mechanical stress. In addition, keratins are involved in establishing apico-basal polarization, regulating motility and cell size and even play a role in complex cellular events such as protein synthesis, membrane traffic or cell signaling.
Keratins are popular diagnostic markers in cancer because of their typical signature for tumor cell type and differentiation, while maintaining the specific expression pattern associated with the cell type of origin. Commonly used markers in the immunohistochemical analysis of tumors are K5-K8 and K18-K20. Adenocarcinomas (epithelial cancers arising in glandular tissues) build up the largest group of human epithelial malignancies. As they can originate in various organs, the ability of differentiating a carcinoma according to the tissue of origin is crucial. By using epithelial keratins as diagnostic markers, the best treatment depending on the exact type of cancer can be determined. In general, most adenocarcinomas express K8, K18 and K19. K7 and K20 levels vary depending on cancer type. In unclear cases, keratin typing is often key to assess the correct tumor type. Beyond their role as diagnostic markers, keratins are also useful prognostic indicators in epithelial malignancies.

The anti-skin layer Keratin sample set provides antibodies directed against Keratin pan, K2e, K10, K14, K8/18 to evaluate the presence and status in IHC and WB.
The set contains enough antibody to perform stainings on 6-12 sections per antibody.

Set content:
Cat. No. GP14S, anti-Keratin Pan guinea pig polyclonal, serum, sample, 600 µl
Cat. No. 65177S, anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant, sample, 600 µl
Cat. No. 11414S, anti-Keratin K10 mouse monoclonal, DE-K10, supernatant, sample, 600 µl
Cat. No. GP-CK14S, anti-Keratin K14 guinea pig polyclonal, serum, sample, 600 µl
Cat. No. GP11S, anti-Keratin K8/K18 guinea pig polyclonal, serum, sample, 600 µl

Reference

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