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IHC of human skin using anti-Perilipin 2 (Cat. No. 610102) (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human skin using anti-Perilipin 2 (Cat. No. 610102) (courtesy of J.Heß, University Hospital Heidelberg)

anti-Perilipin 2,3,5 sample set

The anti-Perilipin 2,3,5 sample set provides antibodies directed against Perilipin 2, 3 and 5 to evaluate the presence and status in IHC and WB.
The set contains enough antibody to perform stainings on 6-12 sections per antibody.

highly_published external_validation
Cat. No.: 70011
Quantity:  600 µl each antibody

 

Cat. No. Product Name Reactivity Application
610102S anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, purified, sample (ready-to-use) dog, human, rat IHC, WB
GP30S anti-Perilipin 3 (N-terminus) guinea pig polyclonal, serum, sample (ready-to-use) bovine, human, mouse, rat IHC, WB
GP31S anti-Perilipin 5 (C-terminus) guinea pig polyclonal, serum, sample (ready-to-use) bovine, human, mouse, rat ICC/IF, IHC, WB

Price as configured: €107.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Number of experiments 6-12 sections per antibody
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use
Western Blot (WB) assay dependent

Background

Details

Lipid droplets (LD) are highly recognized in biomedical research and pathology. These organells are found in nearly all cell types and tissues and the composition of lipidic material varies strongly, depending on their storage or transport function. LDs are linked to several diseases like diabetes, obesity, liposarcoma, atherosclerosis, lipid droplet biogenesis, viral and bacterial infection.   

Perilipins / PAT family proteins

  • 5 subtypes: perilipin 1-5 (PLIN1- PLIN5)
  • located in the membrane of LDs
  • characterization of LD subpopulations and multifunctional properties (lipid transport, lipogenesis and lypolysis)
  • analysis of viral or bacterial infection pathways (targeting LDs)

The anti-Perilipin 2,3,5 sample set provides antibodies directed against Perilipin 2, 3 and 5 to evaluate the presence and status in IHC and WB.
The set contains enough antibody to perform stainings on 6-12 sections per antibody.

Set content:
Cat. No. 610102S, anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, purified, sample, 600 µl
Cat. No. GP30S, anti-Perilipin 3 (N-terminus) guinea pig polyclonal, serum, sample, 600 µl
Cat. No. GP31S, anti-Perilipin 5 (C-terminus) guinea pig polyclonal, serum, sample, 600 µl

Reference

Reference

PublicationSpeciesApplicationAntibody
Gemmink, A. et al. Dissociation of intramyocellular lipid storage and insulin resistance in trained athletes and type 2 diabetes patients; involvement of perilipin 5? J. Physiol. (2017). human WB GP31
Gallardo-Montejano, V. I. et al. Nuclear Perilipin 5 integrates lipid droplet lipolysis with PGC-1a/SIRT1-dependent transcriptional regulation of mitochondrial function. Nat. Commun. 7, (2016). mouse WB, ICC-IF, IP, PLA GP31
Barquissau, V. et al. White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways. Mol. Metab. 5, 352–365 (2016). human WB GP31
Gemmink, A. et al. Decoration of intramyocellular lipid droplets with PLIN5 modulates fasting-induced insulin resistance and lipotoxicity in humans. Diabetologia 59, 1040–8 (2016). human WB, IHC (frozen) GP31
Trevino, M. B. et al. Perilipin 5 regulates islet lipid metabolism and insulin secretion in a cAMP-dependent manner: Implication of its role in the postprandial insulin secretion. Diabetes 64, 1299–1310 (2015). human, mouse WB, ICC-IF GP31
Pourteymour, S. et al. Perilipin 4 in human skeletal muscle: localization and effect of physical activity. Physiol. Rep. 3, (2015). human IHC (paraffin) GP31
Billecke, N. et al. Perilipin 5 mediated lipid droplet remodelling revealed by coherent Raman imaging. Integr. Biol. (Camb). 7, 467–76 (2015). rat IHC (frozen) GP31
Ramos, S. V et al. Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb. Physiol. Reports 2, (2014). rat WB 610102, GP31
Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, (2014). human ICC-IF 610102
Pfisterer, S. G. et al. Lipid droplet and early autophagosomal membrane targeting of Atg2A and Atg14L in human tumor cells. J. Lipid Res. 55, 1267–78 (2014). human WB, ICC-IF 610102
Kuramoto, K. et al. Deficiency of a Lipid Droplet Protein, Perilipin 5, Suppresses Myocardial Lipid Accumulation, Thereby Preventing Type 1 Diabetes- Induced Heart Malfunction. Mol. Cell. Biol.  34, 2721–2731 (2014). mouse WB GP31
Mason, R. R. et al. PLIN5 deletion remodels intracellular lipid composition and causes insulin resistance in muscle. Mol. Metab. 3, 652–663 (2014). mouse IHC (frozen) GP31
Mason, R. R., Meex, R. C. R., Russell, A. P., Canny, B. J. & Watt, M. J. Cellular Localization and Associations of the Major Lipolytic Proteins in Human Skeletal Muscle at Rest and during Exercise. PLoS One 9, (2014). human IHC (frozen) GP31
Straub, B. K. et al. Adipophilin/perilipin-2 as a lipid droplet-specific marker for metabolically active cells and diseases associated with metabolic dysregulation. Histopathology 62, 617–631 (2013). human IHC (paraffin) 610102
Macpherson, R. E. K., Vandenboom, R., Roy, B. D. & Peters, S. J. Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation. Physioligal Reports 1, (2013). rat WB 610102, GP31
MacPherson, R. E. K., Ramos, S. V, Vandenboom, R., Roy, B. D. & Peters, S. J. Skeletal muscle PLIN proteins, ATGL and CGI-58, interactions at rest and following stimulated contraction. Am. J. Physiol. Regul. Integr. Comp. Physiol. 304, R644-50 (2013). rat WB, Co-IP 610102, GP31
Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013). human WB, ICC-IF, IP

610102, GP31, GP30

Dahlhoff, M. et al. PLIN2, the major perilipin regulated during sebocyte differentiation, controls sebaceous lipid accumulation in vitro and sebaceous gland size in vivo NIH Public Access. Biochim. Biophys. Acta 1830, 4642–4649 (2013). human IHC (paraffin) GP31, GP30
Bosma, M. et al. The lipid droplet coat protein perilipin 5 also localizes to muscle mitochondria. Histochem Cell Biol 137, 205–216 (2012). human, rat WB, ICC-IF, IEM GP31
Lin, P. et al. Efficient Lentiviral Transduction of Human Mesenchymal Stem Cells That Preserves Proliferation and Differentiation Capabilities. Stem Cells Transl. Med. 1, 886–897 (2012). human ICC-IF 610102
Timmers, S. et al. Augmenting muscle diacylglycerol and triacylglycerol content by blocking fatty acid oxidation does not impede insulin sensitivity. Proc. Natl. Acad. Sci. U. S. A. 109, 11711–11716 (2012). mouse WB GP31
Chang, P., Khatchadourian, A., Anne McKinney, R. & Maysinger, D. Docosahexaenoic acid (DHA): a modulator of microglia activity and dendritic spine morphology. J. Neuroinflammation 12, (2012). mouse WB GP30
Dichlberger, A. et al. Lipid body formation during maturation of human mast cells. J. Lipid Res. 52, 2198–208 (2011). human WB, ICC-IF 610102, GP30
Macpherson, R. E. K. et al. Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP at rest and following contraction in rat soleus muscle. Am. J. Physiol. - Regul. Integr. Comp. Physiol.  (2011). rat IHC (frozen) GP31
Wang, H. et al. Unique regulation of adipose triglyceride lipase (ATGL) by perilipin 5, a lipid droplet-associated protein. J. Biol. Chem. 286, 15707–15715 (2011). human WB GP31
Timmers, S. et al. Paradoxical Increase in TAG and DAG Content Parallel the Insulin Sensitizing Effect of Unilateral DGAT1 Overexpression in Rat Skeletal Muscle. PLoS One 6, (2011). rat WB GP31
Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480–492 (2010). human WB, IHC 610102, GP30
Ostler, D. A. et al. Adipophilin expression in sebaceous tumors and other cutaneous lesions with clear cell histology: an immunohistochemical study of 117 cases. Mod. Pathol. 23, 567–573 (2010). human IHC (paraffin) 610102
Ingelmo-Torres, M. et al. Hydrophobic and Basic Domains Target Proteins to Lipid Droplets. Traffic 10, 1785–1801 (2009). monkey ICC-IF 610102
Buers, I. et al. TIP47, a Lipid Cargo Protein Involved in Macrophage Triglyceride Metabolism. Arterioscler. Thromb. Vasc. Biol. 29, 767–773 (2009). human WB, ICC-IF 610102, GP30
Cobbe, N. et al. The conserved metalloprotease invadolysin localizes to the surface of lipid droplets. J. Cell Sci. 122, 3414–23 (2009). human WB, ICC-IF GP30
Bulankina, A. V et al. TIP47 functions in the biogenesis of lipid droplets. J. Cell Biol. 185, 641–55 (2009). human WB, ICC-IF GP30
Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936–1946 (2008). human WB, IHC 610102, GP30
Robenek, H. et al. Butyrophilin controls milk fat globule secretion. Proc. Natl. Acad. Sci. USA 103, 10385–10390 (2006). bovine ICC-IF, IEM 610102, GP30
Muthusamy, K., Halbert, G. & Roberts, F. Immunohistochemical staining for adipophilin, perilipin and TIP47. J. Clin. Pathol. 59, 1166–1170 (2006). human IHC (paraffin) 610102, GP30
Robenek, H. et al. Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis. J. Cell Sci. 119, 4215–4224 (2006). human WB 610102
Larigauderie, G. et al. Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of β-oxidation. FEBS J. 273, 3498–3510 (2006). human WB 610102
Yamaguchi, T., Matsushita, S., Motojima, K., Hirose, F. & Osumi, T. MLDP, a novel PAT family protein localized to lipid droplets and enriched in the heart, is regulated by peroxisome proliferator-activated receptor alpha. J. Biol. Chem. 281, 14232–40 (2006). mouse WB, ICC-IF GP31
Hänisch, J., Wältermann, M., Robenek, H. & Steinbüchel, A. Eukaryotic Lipid Body Proteins in Oleogenous Actinomycetes and Their Targeting to Intracellular Triacylglycerol Inclusions: Impact on Models of Lipid Body Biogenesis. Appl. Environ. mircrobiology 72, 6743–6750 (2006). human IEM GP30
Robenek, H., Lorkowski, S., Schnoor, M. & Troyer, D. Spatial Integration of TIP47 and Adipophilin in Macrophage Lipid Bodies. J. Biol. Chem. 280, 5789–5794 (2005). human WB, ICC-IF, IEM 610102, GP30
Robenek, H. et al. Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains. J. Biol. Chem. 280, 26330–26338 (2005). human ICC-IF, IEM 610102, GP30
Robenek, H., Robenek, M. J. & Troyer, D. PAT family proteins pervade lipid droplet cores. J. Lipid Res. 46, 1331–1338 (2005). human IEM 610102, GP30
Ohsaki, Y., Maeda, T. & Fujimoto, T. Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins. Histochem. Cell Biol. 124, 445–452 (2005). human ICC-IF 610102
Fukumoto, S. & Fujimoto, T. Deformation of lipid droplets in fixed samples. Histochem. Cell Biol. 118, 423–8 (2002). human ICC-IF 610102
Fujimoto, T., Kogo, H., Ishiguro, K., Tauchi, K. & Nomura, R. Caveolin-2 Is Targeted to Lipid Droplets, a New " Membrane Domain " in the Cell. J. Cell Biol. 152, 1079–1085 (2001). human WB 610102
Ostermeyer, A. G. et al. Accumulation of Caveolin in the Endoplasmic Reticulum Redirects the Protein to Lipid Storage Droplets. J. Cell Biol. 152, 1071–1078 (2001). human ICC-IF 610102
Buechler, C. et al. Adipophilin is a sensitive marker for lipid loading in human blood monocytes. Biochim. Biophys. Acta 1532, 97–104 (2001). human WB 610102
Hope, R. & McLauchlan, J. Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. J. Gen. Virol. 81, 1913–1925 (2000). syrian hamster WB 610102
Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309–21 (1998). human, rat, dog IHC (frozen), ICC-IF, IEM 610102

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