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human skin (courtesy of J.Heß, University Hospital Heidelberg) Zoom

human skin (courtesy of J.Heß, University Hospital Heidelberg)

anti-Perilipin 2 (mouse N-terminus) guinea pig polyclonal, serum

Adipophilin/Perilipin 2 is an ubiquitous component of lipid droplets, found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines. Detected in the glandular cells of lactating mammary gland, adrenal gland, Sertoli cells, in fat-accumulating hepatocytes. Adipocytes are negative.
Cat. No.: GP42
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€285.00
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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Synthetic peptide (N-terminal aa 1-16 of murine adipophilin, also named PLIN2): MAAAVVDPQQSVVMRV-C
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50 - 1:100
Immunohistochemistry (IHC) - paraffin 1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB) 1:2,000

Background

Details

Adipophilin / ADRP / PLIN2 (a member of the PLIN/PAT family) is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.1998, 2013; Chong et al.).
Polypeptide reacting: Specific for Adipophilin / ADRP / PLIN2, MW 46,646 (calculated from aa sequence data); apparent Mr 50,000 (after SDS-PAGE); pI 6.42.
Tissue immunolocalization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes; adipocytes are negative.
Reactivity on cultured cell lines: 3T3-L1, OP9, HL-1


Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).
Chong, B. M. et al. The adipophilin C terminus is a self-folding membrane-binding domain that is important for milk lipid secretion. J. Biol. Chem. 286, 23254–23265 (2011). Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309–21 (1998).

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