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mouse brain (courtesy of J.Heß, University Hospital Heidelberg) Zoom

mouse brain (courtesy of J.Heß, University Hospital Heidelberg)

anti-Synaptophysin mouse monoclonal, SY38, lyophilized, purified

The anti-synaptophysin antibody SY38 stains neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas and neuroendocrine tumors of epithelial origin, e.g. pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids.
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Cat. No.: 61012
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€225.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone SY38
Immunogen synaptophysin from presynaptic vesicles, prepared from bovine brain
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID P20488
Intended use Research use only
Tested species reactivity bovine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen at least 1:50 with PBS, pH 7.4 (no protease treatment)
Immunohistochemistry (IHC) - paraffin at least 1:50 with PBS, pH 7.4 (microwave treatment recommended, no protease treatment)
Western Blot (WB) assay dependent

Background

Details

SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid. Polypeptide reacting: Mr 38 000 transmembrane glycoprotein of presynaptic vesicles.

Tested cultured cell lines: Rat PC-12 cell line

Reference

Reference

PublicationSpeciesApplication
Hsu, T. M. et al. Hippocampus ghrelin signaling mediates appetite through lateral hypothalamic orexin pathways. Elife 4, (2016). rat IHC (frozen)
Nakajima, C. et al. Low Density Lipoprotein Receptor-related Protein 1 (LRP1) Modulates N-Methyl-D-aspartate (NMDA) Receptor-dependent Intracellular Signaling and NMDA-induced Regulation of Postsynaptic Protein Complexes. J. Biol. Chem. 288, 21909–21923 (2013). mouse WB, ICC-IF
Sato, J., Sasaki, S., Yamada, N. & Tsuchitani, M. Hereditary Cerebellar Degenerative Disease (Cerebellar Cortical Abiotrophy) in Rabbits. Vet. Pathol. 49, 621–628 (2012). rabbit IHC (paraffin)
Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83–93 (2009). human IHC (frozen)
Matsuoka, H. et al. Molecular mechanisms supporting a paracrine role of GABA in rat adrenal medullary cells. J. Physiol. 58620, 4825–4842 (2008). rat ICC-IF
Matsuoka, Y. et al. Impaired adrenocorticotropic hormone response to bacterial endotoxin in mice deficient in prostaglandin E receptor EP1 and EP3 subtypes. Proc. Natl. Acad. Sci. United States Am.  100, 4132–4137 (2003). rat IHC (frozen)
Portela-Gomes, G. M., Stridsberg, M., Johansson, H. & Grimelius, L. Co-localization of synaptophysin with different neuroendocrine hormones in the human gastrointestinal tract. Histochem. Cell Biol. 111, 49–54 (1999). human IHC (paraffin)
Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359–369 (1996). human WB
Leube, R. E. The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108, 883–894 (1995). human, rat WB, ICC-IF
Bennett, M. K., Calakos, N., Kreiner, T. & Scheller, R. H. Synaptic Vesicle Membrane Proteins Interact to Form a Multimeric Complex. J. Cell Biol. 116, 761–775 (1992). rat WB
Dockhorn-Dworniczak, B. et al. Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. Differentiation. 35, 53–71 (1987). human IHC (frozen)
Gould, V. E. et al. Synaptophysin Expression in Neuroendocrine Neoplasms as Determined by Immunocytochemistry. Am. J. Pathol.  126, 243–257 (1987). human IHC (frozen)
Gould, V. E. et al. Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms. Hum. Pathol. 17, 979–83 (1986). human IHC (paraffin)
Wiedenmann, B., Franke, W. W., Kuhn, C., Moll, R. & Gould, V. E. Synaptophysin: a marker protein for neuroendocrine cells and neoplasms. Proc. Natl. Acad. Sci. U. S. A. 83, 3500–4 (1986). human, bovine IHC (frozen)
Wiedenmann, B. & Franke, W. W. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 41, 1017–28 (1985). mouse, bovine WB, IHC (frozen)

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