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Synaptopodin staining in human glomeruli (GP94-C; dilution 1:50;  Image courtesy of A.Weins, Brigham and Women's Hospital) Zoom

Synaptopodin staining in human glomeruli (GP94-C; dilution 1:50; Image courtesy of A.Weins, Brigham and Women's Hospital)

anti-Synaptopodin (C-terminus) guinea pig polyclonal, serum

The antibody reacts specifically with synaptopodin and recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. GP94-C also reacts with a subset of exclusively telencephalic synapses.
Cat. No.: GP94-C
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€299.00
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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptides (mouse C-terminus), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse
Note centrifuge prior to opening
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:50 – 1:100
Immunohistochemistry (IHC) - paraffin 1:50 – 1:100 (microwave treatment recommended)
Western Blot (WB) 1:500

Background

Details

The antibody reacts specifically with the C-terminus of synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding pro-teins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corre-sponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to posttransla-tional modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.

The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin.
Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentiation-dependent expression in cultured hippocampal neurons.

Reference

Reference

PublicationSpeciesApplication
Siegerist, F. et al. Novel Microscopic Techniques for Podocyte Research. Front.Endocrinol.(Lausanne). 9, 379 (2018). mouse, rat IHC-IF (paraffin)

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