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rat kidney (courtesy of J. Heß, University Hospital Heidelberg) Zoom

rat kidney (courtesy of J. Heß, University Hospital Heidelberg)

anti-Synaptopodin mouse monoclonal, G1D4, lyophilized, purified

The anti-synaptopodin antibody recognizes differentiated glomerular visceral epithelial cells (podocytes) in vivo and in vitro. Weaker additional reaction with arterial endothelial cells.
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Cat. No.: 61094
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€291.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone G1D4
Immunogen Isolated rat kidney glomeruli
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID Q9Z327
Tested species reactivity bovine, gerbil, guinea pig, human, mouse, rat (not with rabbit, frog, chicken)
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:5 – 1:10
Immunohistochemistry (IHC) - paraffin 1:5 – 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with α-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. The antibody reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.

Reference

Reference

PublicationSpeciesApplication
Haley, K. et al. Podocyte injury elicits loss and recovery of cellular forces. Sci.Adv. 4, eaap8030 (2018). human ICC
Endlich, N. et al. The transcription factor Dach1 is essential for podocyte function. J.Cell.Mol.Med. 22, 2656-2669 (2018). human, mouse ICC
Jain, N. et al. TLR-mediated albuminuria needs TNFα-mediated cooperativity between TLRs present in hematopoietic tissues and CD80 present on non-hematopoietic tissues in mice. Dis. Model. Mech. 9, 707–17 (2016). mouse IHC (frozen)
Wang, L. et al. Gq signaling causes glomerular injury by activating TRPC6. J. Clin. Invest. 125, 1913–26 (2015). mouse WB, IHC (frozen)
Ihara, K. I. et al. MAGI-2 is critical for the formation and maintenance of the glomerular filtration barrier in mouse kidney. Am. J. Pathol. 184, 2699–2708 (2014). mouse IHC (frozen)
Tanaka, E. et al. Notch2 activation ameliorates nephrosis. Nat. Commun. 5, 3296 (2014). mouse IHC (frozen)
Wagner, N. et al. The podocyte protein nephrin is required for cardiac vessel formation. Hum. Mol. Genet. 20, 2182–2194 (2011). mouse IHC (frozen)
Krall, P. et al. Podocyte-Specific Overexpression of Wild Type or Mutant Trpc6 in Mice Is Sufficient to Cause Glomerular Disease. PLoS One 5, (2010). mouse IHC (frozen)
Matsusaka, T. et al. ARB Protection Against Podocyte-Induced Sclerosis is Podocyte AT1-Independent. Hypertension 55, 967–973 (2010). mouse IHC
Turk, T. et al. BMP Signaling and Podocyte Markers Are Decreased in Human Diabetic Nephropathy in Association With CTGF Overexpression. J. Histochem. Cytochem. 57, 623–631 (2009). human, mouse IHC (paraffin)
Walter, B., Schlechter, T., Hergt, M., Berger, I. & Hofmann, I. Differential expression pattern of protein ARVCF in nephron segments of human and mouse kidney. Histochem. Cell Biol. 130, 943–956 (2008). human IHC (frozen)
Koop, K. et al. Selective Loss of Podoplanin Protein Expression Accompanies Proteinuria and Precedes Alterations in Podocyte Morphology in a Spontaneous Proteinuric Rat Model. Am. J. Pathol. 173, 315–326 (2008). rat IHC (paraffin)
Wagner, K.-D. et al. An Inducible Mouse Model for PAX2-Dependent Glomerular Disease: Insights into a Complex Pathogenesis. Curr. Biol. 16, 793–800 (2006). human IHC
Schiwek, D. et al. Stable expression of nephrin and localization to cell-cell contacts in novel murine podocyte cell lines. Kidney Int. 66, 91–101 (2004). mouse ICC-IF
Peitsch, W. K. Cell biological and biochemical characterization of drebrin complexes in mesangial cells and podocytes of renal glomeruli. J. Am. Soc. Nephrol. 14, 1452–1463 (2003). human, mouse, rat, bovine IHC (frozen), IEM
Patek, C. E. et al. Murine Denys–Drash syndrome: evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis. Hum. Mol. Genet. 12, 2379–2394 (2003). mouse IHC (paraffin)
Barisoni, L., Kriz, W., Mundel, P. & D’Agati, V. The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy. J. Am. Soc. Nephrol. 10, 51–61 (1999). human IHC (paraffin)
Kihara, I. et al. Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy. Histopathology 34, 537–47 (1999). human IHC (paraffin)
Mundel, P., Reiser, J. & Kriz, W. Induction of differentiation in cultured rat and human podocytes. J. Am. Soc. Nephrol.  8, 697–705 (1997). human, mouse ICC-IF
Mundel, P. et al. Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines. Exp. Cell Res. 236, 248–58 (1997). mouse ICC-IF
Mundel, P. et al. Synaptopodin: An Actin-associated Protein in Telencephalic Dendrites and Renal Podocytes. J. Cell Biol. 139, 193–204 (1997). rat WB, IHC (frozen), IEM
Mundel, P., Gilbert, P. & Kriz, W. Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 KD Protein’. J. Histochem. Cytochem. 39, 1047–1056 (1991). human, rat IHC (frozen), IEM

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