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Mouse cerebellum section stained with chicken anti-MAP 2 and protag-HiSec anti-chicken IgY-X2 Sulfo-Cyanin 5 (Cy5)(Cat. No. 80410; green). Nuclei were stained using DAPI (blue)(courtesy of NanoTag Biotechnologies GmbH). Zoom

Mouse cerebellum section stained with chicken anti-MAP 2 and protag-HiSec anti-chicken IgY-X2 Sulfo-Cyanin 5 (Cy5)(Cat. No. 80410; green). Nuclei were stained using DAPI (blue)(courtesy of NanoTag Biotechnologies GmbH).

protag-HiSec anti-chicken IgY-X2 - select your fluorophore

Camelid single-domain antibody (sdAb) directed against chicken IgY. The single-domain antibody is labeled with 2 molecules of fluorophore.
Cat. No.: 804xx
Quantity:  500 µl

Delivery Time: usually 1-7 working days

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Product description

Host llama/ alpaca
Antibody Type recombinant, produced in E.coli
Isotype single-domain antibody
Immunogen chicken IgY
Purification affinity chromatography
Formulation 5 μM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide.
Storage up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity chicken IgY (no cross-reaction with mouse, rat, rabbit, guinea pig, donkey, goat, pig, horse, cow or human IgGs)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500

Background

Details

protag-HiSec anti-chicken IgY is an isotype- and species-specific camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It is directed against chicken IgY and contains two site-specifically coupled fluorophores per protag molecule. One single-domain antibody targets two fluorophores to a primary chicken antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. There are no batch-to-batch variations, since the protag-HiSecs single-domain antibodies are recombinantly expressed.

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