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Product description
| Host | llama/ alpaca |
|---|---|
| Antibody Type | recombinant, produced in E.coli |
| Isotype | single-domain antibody |
| Clone | 1H7 |
| Immunogen | TagFP |
| Purification | affinity chromatography |
| Formulation | 50 % slurry in PBS containing 20 % Ethanol |
| Storage | store at 2-8°C |
| No. of experiments | up to 100 reactions |
| Intended use | Research use only |
| Tested species reactivity | mKate, mKate2, mTagBFP, mTagRFP, mTagRFP657 (does not cross-react with Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, dsRed or their most common derivatives) |
| Support | 4 % cross-linked agarose, bead size 50-150 μm |
| Coating | sdAb anti-TagFP clone 1H7 |
| Capacity | > 4 μg mTagBFP per μl of packed beads |
| Stability | • Common buffer substances at pH 5 to 9 • 2% Triton X-100, 1% Tween-20, 1% NP-40, 1% CHAPS, 1% Deoxycholate, 0.1% SDS • 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA • 4 M urea • 10 mM DTT, 10 mM 2-Mercaptoethanol • RNAse A, DNAse I, Benzonase, protease inhibitors |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunoprecipitation (IP) | yes |
Background
Details
protag-HiPur TagFP Agarose Beads are based on a high-affinity single-domain antibodies (sdAb) that are covalently immobilized on 4% cross-linked agarose beads. The sdAbs are attached via a flexible linker which guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. protag-HiPur TagFP Agarose Beads thus feature high affinity and superior capacity for TagFP fusion proteins while showing negligible non-specific background. protag-HiPur TagFP Agarose Beads immobilize a wide range of TagFP derivatives, such as mTagBFP, mTagRFP, mTagRFP657 and mKate. Reactivity against other TagRFP derivatives is not analyzed. It does not cross-react with common GFP- or dsRed/RFP derivatives including EBFP. protag-HiPur GFP Agarose Beads are compatible not only with physiological buffers but also with high stringency buffers. With protag-HiPur TagFP Agarose Beads the binding and washing conditions can be adjusted to the experimental needs.
Reference
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