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Stable HeLa cell line expressing YFP-fusion protein (A, false color). Cells were stained with protag-HiRes anti-GFP-X1 Atto 542 (B, false color, Cat. No. 81106). Overlay of both channels and additional DAPI stain (C)(courtesy of NanoTag Biotechnologies Gm Zoom

Stable HeLa cell line expressing YFP-fusion protein (A, false color). Cells were stained with protag-HiRes anti-GFP-X1 Atto 542 (B, false color, Cat. No. 81106). Overlay of both channels and additional DAPI stain (C)(courtesy of NanoTag Biotechnologies Gm

protag-HiRes anti-GFP-X1 Abberior® Star 635P, large

Camelid single-domain antibody (sdAb) directed against GFP. The single-domain antibody is labeled with 1 molecule of Abberior® Star 635P.
Cat. No.: 81104L
Quantity:  200 µl

Delivery Time: usually 1-7 working days

$504.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host llama/ alpaca
Antibody Type recombinant, produced in E.coli
Isotype single-domain antibody
Clone 1H1
Immunogen GFP
Purification affinity chromatography
Conjugate Abberior® Star 635P
Formulation 5 μM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide.
Storage up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity acGFP, Cerulean, Citrine, eCFP, EGFP, Emerald GFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, Sirius, superecliptic pHluorin, superfold GFP, tSapphire (does not cross-react with Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:1000

Background

Details

protag-HiRes anti-GFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise ½, acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus, Citrine.

In protag-HiRes anti-GFP-X1, each fluorophore is coupled to exactly one single-domain antibody, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

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