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COS cells transfected with mScarlet-i-tubullin (A) and stained with protag-HiRes anti-mScarlet-i-X2 Atto 488 (B, Cat. No. 84205). Overlay in (C)(courtesy of NanoTag Biotechnologies GmbH). Zoom

COS cells transfected with mScarlet-i-tubullin (A) and stained with protag-HiRes anti-mScarlet-i-X2 Atto 488 (B, Cat. No. 84205). Overlay in (C)(courtesy of NanoTag Biotechnologies GmbH).

protag-HiRes anti-mScarlet-i-X2 Atto 488

Camelid single-domain antibody (sdAb) directed against mScarlet-i. The single-domain antibody is labeled with 2 molecules of Atto 488.
Cat. No.: 84205
Quantity:  20 µl

Delivery Time: usually 1-7 working days

$145.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host llama/ alpaca
Antibody Type recombinant, produced in E.coli
Isotype single-domain antibody
Clone 2B12
Immunogen RFP
Purification affinity chromatography
Conjugate Atto 488
Formulation 2.5 μM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide.
Storage up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity dsRed1, dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato (does not cross-react with Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, mTagBFP or their most common derivatives)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500

Background

Details

protag-HiRes anti-mScarlet-i camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes mScarlet-i in its native conformation with high affinity and specificity. It can also recognize some mRFP-derived red fluorescent protein like mOrange2, dsRed, tdTomato, mRFP and mCherry in its native conformation. It does not cross-react with GFP or mTagBFP derivatives.

In protag-HiRes anti-mScarlet-i-X2, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. protag-HiRes anti-mScarlet-i-X2 can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, the protag-HiRes anti-mScarlet-i-X2 can thus improve the localization accuracy by 10-15 nm. Both features – enhanced brightness and precise fluorophore placement – render the protag-HiRes anti-mScarlet-i-X2 products superior tools for all microscopy techniques.

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