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3T3 cells transfected with the mCherry-tubulin were stained with protag-HiRes anti-RFP-X1 Atto 542 (false color, Cat. No. 81106)(courtesy of NanoTag Biotechnologies GmbH). Zoom

3T3 cells transfected with the mCherry-tubulin were stained with protag-HiRes anti-RFP-X1 Atto 542 (false color, Cat. No. 81106)(courtesy of NanoTag Biotechnologies GmbH).

protag-HiRes anti-RFP-X1 Abberior® Star 635P, large

Camelid single-domain antibody (sdAb) directed against RFP. The single-domain antibody is labeled with 1 molecule of Abberior® Star 635P.
Cat. No.: 82104L
Quantity:  200 µl

Delivery Time: usually 1-7 working days

$504.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host llama/ alpaca
Antibody Type recombinant, produced in E.coli
Clone 2B12
Immunogen RFP
Purification affinity chromatography
Conjugate Abberior® Star 635P
Formulation 5 μM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide.
Storage up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity dsRed1, dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato (does not cross-react with Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, mTagBFP or their most common derivatives)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:1000

Background

Details

protag-HiRes anti-RFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes RFP and its most common derivatives with high affinity and specificity. It recognizes mOrange2, dsRed1, dsRed2, tdTomato, mRFP, mCherry, mScarlet-i.

In protag-HiRes anti-RFP-X1, each fluorophore is coupled to exactly one single-domain antibody, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

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