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WB with anti-AAV5 antibody (Cat. No. 610137, 1:50) and secondary anti-rabbit HRP antibody (Cat. No. 90003, 1:10,000), sample AAV5 capsids (75-400 ng) Zoom

WB with anti-AAV5 antibody (Cat. No. 610137, 1:50) and secondary anti-rabbit HRP antibody (Cat. No. 90003, 1:10,000), sample AAV5 capsids (75-400 ng)

anti-rabbit IgG goat polyclonal, HRP conjugate

Whole rabbit IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Cat. No.: 90003
Quantity:  100 µg

Delivery Time: usually 1-7 working days

$109.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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Product description

Host goat
Antibody Type polyclonal
Immunogen rabbit IgG
Purification affinity chromatography
Conjugate peroxidase/ HRP
Formulation lyophilized, reconstitute in 500 µL dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity rabbit

Applications

Tested applications Tested dilutions
ELISA 1:1,250 - 1:25,000
Western Blot (WB) 1:2,500 - 1:50,000

Background

Details

Whole rabbit IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.

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