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Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
910HISL |
anti-6-His-tag mouse monoclonal, 6His |
lyophilized, purified, large | 100 µg |
$398.00
Excl. VAT, excl. Shipping Cost
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | 6His |
Immunogen | recombinant protein containing the sequence HHHHHH |
Purification | affinity chromatography |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 100 µl sterile PBS, pH 7.4 (final concentration 0.25 mg/ml) |
Storage | lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles) |
No. of experiments | ≤ 100 (WB, 5 ml reaction volume) |
Intended use | Research use only |
Tested species reactivity | 6-His-tag |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunoprecipitation (IP) | assay dependent |
Western Blot (WB) | 1:2,000-1:5,000 (0.125 – 0.05 µg/ml) |
Background
Details
The monoclonal 6-His antibody recognizes polyhistidine (6-His). The 6-His tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Reference
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