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WB with anti-Keratin K13 antibody (Cat. No. 61007, 1:50), MCF7 whole cell lysate (5-10 µg) Zoom

WB with anti-Keratin K13 antibody (Cat. No. 61007, 1:50), MCF7 whole cell lysate (5-10 µg)

anti-Keratin K13, Ks13.1 + positive western blot control

The set provides anti-Keratin K13, Ks13.1 antibody and associated western blot control - positively tested MCF7 cell lysate.
The set contains enough western blot control to perform 5 western blots.
The anti-keratin K13 Ks13.1 antibody reacts with non-cornified squamous epithelia and neoplasms derived therefrom. Protease pretreatment improves the performance of this excellent marker in immunohistochemistry.

Cat. No.: 61007-WBC
Quantity:  50 µg anti-Keratin K13 + 50 µg western blot control

 

Cat. No. Product Name Reactivity Application
61007 anti-Keratin K13 mouse monoclonal, Ks13.1, lyophilized, purified bovine, human, rat ICC/IF, IHC, WB
64010 Positive western blot control: anti-Keratin K13 antibody WB

Price as configured: €241.00

Excl. Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks13.1
Immunogen Keratin K13 of Mr 54 000 purified from human esophagus
Purification cell culture lysate, protein A affinity chromatography
Conjugate unconjugated
Formulation antibody: lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4); western blot control: lyophilized, reconstitute in 50 µL 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)
Storage antibody: short term at 2 – 8 °C; long term in aliquots at - 20 °C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components
Tested species reactivity bovine, human, rat
Intended use research use only

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) anti-Keratin K13: assay dependent
Immunohistochemistry (IHC) - frozen anti-Keratin K13: 1:10
Immunohistochemistry (IHC) - paraffin anti-Keratin K13: 1:10 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K13: 1:50 - 1:100; western blot control: 5-10 µg total protein per lane

Background

Details

anti-Keratin K13 antibody:
Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin.
Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13; with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000 and also to K25, formerly designated K25irs1 from inner root sheath of hair follicle).
Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma
Reactivity on cultured cell lines: Cell lines from squamous cell CA, e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder CA

Positive western blot control:
Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.
MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.

Set content:
Cat. No. 61007, anti-Keratin K13 mouse monoclonal, Ks13.1, lyophilized, purified
Cat. No. 64010, Positive western blot control: anti-Keratin K13 antibody

Reference

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