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WB with anti-Keratin K19 antibody (Cat. No. 61029, 1:100), MCF7 whole cell lysate (5-10 µg) Zoom

WB with anti-Keratin K19 antibody (Cat. No. 61029, 1:100), MCF7 whole cell lysate (5-10 µg)

anti-Keratin K19, Ks19.2 (Z105.6) + positive western blot control

The set provides anti-Keratin K19, Ks19.2 (Z105.6) antibody and associated western blot control - positively tested MCF7 cell lysate.
The set contains enough western blot control to perform 5 western blots.
The anti-Keratin K19 antibody represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma.

Cat. No.: 61029-WBC
Quantity:  50 µg anti-Keratin K19 + 50 µg western blot control

 

Cat. No. Product Name Reactivity Application
61029 anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), lyophilized, purified bovine, human, rabbit, rat ELISA, ICC/IF, IHC, WB
64008 Positive western blot control: anti-Keratin K19 antibody WB

Price as configured: $248.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone Ks19.2 (Z105.6) (also published as BM 19.21, MAK 19.21)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Purification cell culture lysate, protein A affinity chromatography
Conjugate unconjugated
Formulation antibody: lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4); western blot control: lyophilized, reconstitute in 50 µL 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)
Storage antibody: short term at 2 – 8 °C; long term in aliquots at - 20 °C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components
Intended use research use only
Tested species reactivity bovine, human, rabbit, rat ((negative with mouse, woodchuck, chicken, xenopus))

Applications

Tested applications Tested dilutions
ELISA anti-Keratin K19: assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) anti-Keratin K19: assay dependent
Immunohistochemistry (IHC) - frozen anti-Keratin K19: 1:10 -1:50
Immunohistochemistry (IHC) - paraffin anti-Keratin K19: 1:10 -1:50 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K19: 1:50 - 1:500; western blot control: 5-10 µg total protein per lane

Background

Details

anti-Keratin K19 antibody:
Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma! Polypeptide Reacting: Mr 40 000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the α-helical fragment.
Tumors specifically detected: All tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma.
Reactivity on cultured cell lines: MCF-7

Positive western blot control:
Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.
MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.

Set content:
Cat. No. 61029, anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), lyophilized, purified
Cat. No. 64008, Positive western blot control: anti-Keratin K19 antibody

Reference

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