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WB with anti-Keratin K8 antibody (Cat. No. 61038, 1:100), MCF7 whole cell lysate (5-10 µg) Zoom

WB with anti-Keratin K8 antibody (Cat. No. 61038, 1:100), MCF7 whole cell lysate (5-10 µg)

anti-Keratin K8, Ks8.7 + positive western blot control

The set provides anti-Keratin K8, Ks8.7 antibody and associated western blot control - positively tested MCF7 cell lysate.
The set contains enough western blot control to perform 5 western blots.
The anti-Keratin K8 antibody recognizes all simple-type epithelia and neoplasms derived therefrom. Stains all adenocarcinomas tested and undifferentiated carcinomas.

application_confirmed highly_published external_validation validated_by_epitope_mapping
Cat. No.: 61038-WBC
Quantity:  50 µg anti-Keratin K8 + 50 µg western blot control

 

Cat. No. Product Name Reactivity Application
61038 anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified hamster, human ICC/IF, IHC, WB
64009 Positive western blot control: anti-Keratin K8 antibody WB

Price as configured: €241.00

Excl. Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Ks8.7
Immunogen cytoskeletal proteins from cultured HeLa cells
Purification cell culture lysate, protein A affinity chromatography
Conjugate unconjugated
Formulation antibody: lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4); western blot control: lyophilized, reconstitute in 50 µL 1 x SDS buffer (lysis buffer composition: PBS + Pefablock)
Storage antibody: short term at 2 – 8 °C; long term in aliquots at - 20 °C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components
Intended use research use only
Tested species reactivity hamster, human ((negative with bovine, pig))

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) anti-Keratin K8: assay dependent
Immunohistochemistry (IHC) - frozen anti-Keratin K8: 1:50 - 1:100
Immunohistochemistry (IHC) - paraffin anti-Keratin K8: 1:50 - 1:100 (microwave treatment recommended)
Western Blot (WB) anti-Keratin K8: 1:50 - 1:500; western blot control: 5-10 µg total protein per lane

Background

Details

anti-Keratin K8 antibody:
Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin.
Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia.
Reactivity on cultured cell lines: MCF-7
Tumors specifically detected: All adenocarcinoma tested; undifferentiated carcinoma; cervix carcinoma; hepatocellular carcinoma

Positive western blot control:
Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.
MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.

Set content:
Cat. No. 61038, anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purified
Cat. No. 64009, Positive western blot control: anti-Keratin K8 antibody

Reference

Reference

PublicationSpeciesApplication
Larribere, L. et al. An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. Stem.Cell.Reports. 9, 1234-1245 (2017). human IHC-IF (paraffin)
Bitam, S. et al. An unexpected effect of TNF-α on F508del-CFTR maturation and function. F1000 Res. 4, (2015). human ICC-IF
Zatloukal, B. et al. Sensitivity and Specificity of In situ Proximity Ligation for Protein Interaction Analysis in a Model of Steatohepatitis with Mallory-Denk Bodies. PLoS One 9, (2014). mouse IHC (frozen)
Ito, K. et al. Loss of Runx3 Is a Key Event in Inducing Precancerous State of the Stomach. Gastroenterology 140, 1536–1546.e8 (2011). mouse IHC (paraffin)
Plum, W. et al. Lack of Glycoprotein 130/Signal Transducer and Activator of Transcription 3-Mediated Signaling in Hepatocytes Enhances Chronic Liver Injury and Fibrosis Progression in a Model of Sclerosing Cholangitis. Am. J. Pathol. 176, 2236–2246 (2010). mouse IHC (frozen)
Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005). human IHC (frozen)
Zatloukal, K. et al. p62 Is a Common Component of Cytoplasmic Inclusions in Protein Aggregation Diseases. Am. J. Pathol. 160, 255–263 (2002). human ICC-IF
Stumptner, C., Fuchsbichler, A., Heid, H., Zatloukal, K. & Denk, H. Mallory body—A disease-associated type of sequestosome. Hepatology 35, 1053–1062 (2002). human, mouse IHC (frozen), IEM

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