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Product description
| Conjugate | peroxidase/ HRP |
|---|---|
| Storage | store at 2 – 8 °C |
| Number of experiments | 75-150 sections |
| Intended use | research use only |
| Tested species reactivity | guinea pig |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | yes |
| Immunohistochemistry (IHC) - paraffin | yes |
Background
Details
The prohisto-Set anti-guinea pig HRP + DAB duo provides prohisto-HiSec anti-guinea pig HRP and DAB duo staining solution.
Set content:
Cat. No. PRSTAG, prohisto-HiSec anti-guinea pig HRP
Cat. No. PRDAB1, prohisto-HiVis DAB duo
The prohisto-HiSec anti-guinea pig HRP is used for staining of tissue sections or cells in IHC and ICC applications. It is a highly sensitive enzymatic, non-biotin, one-step detection kit which produces minimal background staining.
The prohisto-HiSec detection system uses novel conjugation and micropolymer chemistries. The highly active peroxidase micropolymer is attached to affinity purified secondary antibodies with outstanding sensitivity and low background.
Steric interference of prohisto-HiSec peroxidase polymers is limited and accessibility to the target is enhanced. The result is distinct, strong staining of antibody targets with great sensitivity.
The prohisto-HiVis DAB duo is used for the staining of tissue sections or cells, or for use on nylon or PVDF membranes.
The kit contains two stock solutions, Reagent A (15 ml) and Reagent B (15 ml) are mixed together in equal volumes to receive the working solution. prohisto-HiVis DAB duo produce a brown reaction product withing 3 to 5 minutes in presence of peroxidase (HRP) enzyme. The working solution is stable for several days when stored at 4°C.
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