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IHC of bovien kidney using mouse anti-Keratin K18 antibody (Cat. No. 61028) and prohisto-HiVis DAB duo Zoom

IHC of bovien kidney using mouse anti-Keratin K18 antibody (Cat. No. 61028) and prohisto-HiVis DAB duo

prohisto-Set anti-mouse HRP + DAB duo

The prohisto-Set anti-mouse HRP + DAB duo provides prohisto-HiSec anti-mouse HRP and DAB duo staining solution.
Cat. No.: PRDABM
Number of experiments:  75-150 sections
Quantity:  15 ml anti-mouse HRP + 30 ml DAB

 

Cat. No. Product Name Reactivity Application
PRSTAM prohisto-HiSec anti-mouse HRP mouse ICC/IF, IHC
PRDAB1 prohisto-HiVis DAB duo ICC/IF, IHC

Price as configured: €206.00

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Product description

Conjugate peroxidase/ HRP
Storage store at 2 – 8 °C
Tested species reactivity mouse
Intended use research use only
Number of experiments 75-150 sections

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) yes
Immunohistochemistry (IHC) - frozen yes
Immunohistochemistry (IHC) - paraffin yes

Background

Details

The prohisto-Set anti-mouse HRP + DAB duo provides prohisto-HiSec anti-mouse HRP and DAB duo staining solution.

Set content:
Cat. No. PRSTAM, prohisto-HiSec anti-mouse HRP
Cat. No. PRDAB1, prohisto-HiVis DAB duo

The prohisto-HiSec anti-mouse HRP is used for staining of tissue sections or cells in IHC and ICC applications. It is a highly sensitive enzymatic, non-biotin, one-step detection kit which produces minimal background staining.

The prohisto-HiSec detection system uses novel conjugation and micropolymer chemistries. The highly active peroxidase micropolymer is attached to affinity purified secondary antibodies with outstanding sensitivity and low background.
Steric interference of prohisto-HiSec peroxidase polymers is limited and accessibility to the target is enhanced. The result is distinct, strong staining of antibody targets with great sensitivity.

The prohisto-HiVis DAB duo is used for the staining of tissue sections or cells, or for use on nylon or PVDF membranes.
The kit contains two stock solutions, Reagent A (15 ml) and Reagent B (15 ml) are mixed together in equal volumes to receive the working solution. prohisto-HiVis DAB duo produce a brown reaction product withing 3 to 5 minutes in presence of peroxidase (HRP) enzyme. The working solution is stable for several days when stored at 4°C.



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