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WB with anti-Keratin K13 antibody (Cat. No. 61007, 1:50), MCF7 whole cell lysate (5-10 µg) western blot control Zoom

WB with anti-Keratin K13 antibody (Cat. No. 61007, 1:50), MCF7 whole cell lysate (5-10 µg) western blot control

AAV1 ELISA Control

The AAV1 ELISA Control consists of fully assembled, empty AAV1 capsids. It represents the serotype-specific conformational epitopes and is therefore a reliable positive control in detection and quantification assays based on AAV antibodies detecting the conformational epitopes, such as ELISA.
Cat. No.: PRAAV1-C
Quantity:  1 vial (0.6E+09 – 1.0E+09 capsids)

Delivery Time: usually 1-7 working days

$135.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Alternative Products

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Product description

Formulation Lyophilized, empty AAV1 capsids. Reconstitute in 500 μl ASSB 1x (provided with PROGEN’s AAV1 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing!
Storage store at 2-8°C
Concentration

1.2E+09 – 2.0E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial)

Intended use Research use only
Stability 4 weeks at 2-8°C after reconstitution in ASSB 1x

Applications

Tested applications Tested dilutions
ELISA As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN’s AAV1 Titration ELISA) and analysis at least in duplicates is recommended.

Background

Details

The AAV1 ELISA Control consists of fully assembled, empty AAV1 capsids. The concentration is lot specific.

The AAV1 ELISA Control can be used as a positive control with PROGEN’s AAV1 Titration ELISA (PRAAV1). If you require different lots of the AAV1 ELISA Control and the Kit Control included in your PROGEN AAV1 Titration ELISA, please enquire to check availability.

Reading of the AAV1 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN’s AAV1 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.

The AAV1 ELISA Control has been calibrated on an internally established reference standard. The internal reference standard is a preparation of full AAV1 capsids that has been characterized by qPCR (DNA quantification) and electron microscopy (ratio of full to empty capsids). For further information, please see as an example our poster ‘The New AAV3 Titration ELISA’ available on aavvirus.com.

Publications

Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17–24 (2007).

Reference

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