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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| 61058 |
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
lyophilized, purified | 50 µg |
$450.00
Excl. VAT, excl. Shipping Cost
|
| 65158 |
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
supernatant | 5 mL |
$265.00
Excl. VAT, excl. Shipping Cost
|
| 61058S |
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
lyophilized, purified, sample | 10 µg |
$128.00
Excl. VAT, excl. Shipping Cost
|
| 61058-647 |
Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
AFDyeTM 647 Conjugate | 50 µg (100 µl) |
$498.00
Excl. VAT, excl. Shipping Cost
|
Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | B1 |
| Immunogen | AAV2 capsids |
| Purification | protein A affinity chromatography |
| Conjugate | AFDyeTM 488 |
| Formulation | liquid; PBS + 0.09% sodium azide |
| Storage | up to 1 month: 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles; store in the dark. |
| Intended use | Research use only |
| Tested species reactivity | AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Western Blot (WB) | 1:2,500-1:5,000 (0.1-0.2 µg/ml; detection limit 5E+09 capsids) |
Background
Details
The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.
The AFDye™ 488 is a green emitting dye, structurally and spectrally equivalent to Alexa Fluor® 488 and therefore ideally suited for the 488 nm laser line (excitation/emission 494/517 nm). It can be used with a common FITC filter set.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
Reference
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