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WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 61058, 1:250) using AAV2 capsids as sample Zoom

WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 61058, 1:250) using AAV2 capsids as sample

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, lyophilized, purified, sample

Useful for immunolocalization studies of capsid formation in combination with Cat. No. 61055 and rabbit anti-AAV (Cat. No. 61084). Reacts with free VP1, VP2 and VP3 and at a very reduced level with assembled capsids. Recognizes all published AAV serotypes (AAV4, 11 & 12, no cross-reaction)
Cat. No.: 61058S
Quantity:  10 µg

Delivery Time: usually 1-7 working days

$128.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Alternative Products

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61058

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1

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Excl. 19% Tax, excl. Shipping Cost
65158

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone B1
Immunogen adeno-associated virus 2 capsid proteins and virus particles
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 200 µl sterile PBS
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity AAV2 (published cross-reactivity with all published AAV serotypes (AAV4, 11 &12, no cross-reaction))

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent (mainly free VP proteins)
Western Blot (WB) 1:250

Background

Details

Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. 61055/ 65155) and rabbit poly-clonal antibody to AAV capsid proteins (Cat. No. 61084). B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al. 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Reference

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