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WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 61058, 1:250) using AAV2 capsids as sample Zoom

WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 61058, 1:250) using AAV2 capsids as sample

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, lyophilized, purified

Useful for immunolocalization studies of capsid formation in combination with Cat. No. 61055 and rabbit anti-AAV (Cat. No. 61084). Reacts with free VP1, VP2 and VP3 and at a very reduced level with assembled capsids. Recognizes all published AAV serotypes (AAV4, 11 & 12, no cross-reaction)
Cat. No.: 61058
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$450.00
Excl. VAT, excl. Shipping Cost

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Specifications

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone B1
Immunogen AAV2 capsids
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml sterile PBS
Storage lyophilized antibody: 2-8°C; reconstituted antibody: short term at 2-8°C; for long term storage we recommend to add 0.5% BSA prior to storing in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Tested species reactivity AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
Dot Blot 1:500 (0.1 µg/ml; denaturing conditions)
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunoprecipitation (IP) assay dependent (precipitation of mainly free VP proteins)
Western Blot (WB) 1:250 - 1:500 (0.1 - 0.2 µg/ml)

Background

Details

B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al. 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Reference

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