protag-HiPur RFP Agarose Beads

Cat. No. 89200L

Available, delivery time 1-7 days

€546.00*

Key Features
Camelid single-domain antibody (sdAb) covalently linked to 4% agarose beads.
Product description
Quantity 2 ml
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Application IP
Purification Affinity chromatography
Reactivity dsRed1/dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, mTagBFP or their most common derivatives
Storage 2-8°C
Intended use Research use only
Clone 2B12
Immunogen RFP
Formulation 50% slurry in PBS containing 20% Ethanol
Capacity > 4 µg RFP per µl of packed beads
Coating sdAb anti-RFP clone 2B12
Support 4% cross-linked agarose, bead size 50-150 µm
Buffer compatibility
  • Common buffer substances at pH 5 to 9
  • 2% Triton X-100, 1% Tween-20, 1% NP-40, 1% CHAPS, 1% Deoxycholate, 0.1% SDS
  • 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
  • 4 M urea
  • 10 mM DTT, 10 mM 2-Mercaptoethanol   
  • RNAse A, DNAse I, Benzonase, protease inhibitors
Applications
Tested applications Tested dilutions
Immunoprecipitation (IP) yes
Background
protag-HiPur RFP Agarose Beads are based on a high-affinity single-domain antibodies (sdAb) that are covalently immobilized on 4% cross-linked agarose beads. The sdAbs are attached via a flexible linker which guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. protag-HiPur RFP Agarose Beads thus feature high affinity and superior capacity for RFP fusion proteins while showing negligible non-specific background. protag-HiPur GFP Agarose Beads immobilize a wide range of RFP derivatives, such as most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato and mScarlet. It does not cross-react with GFP or mTagBFP/mTagRFP derivatives. protag-HiPur RFP Agarose Beads are compatible not only with physiological buffers but also with high stringency buffers. With protag-HiPur RFP Agarose Beads the binding and washing conditions can be adjusted to the experimental needs.
Downloads
File
Category
Size
Filetype
File IP
Category Protocol
Size 831.95 KB
Filetype pdf
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