protag-HiRes anti-RFP-X1 Atto 542

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Cat. No. 82106L

Available, delivery time 1-7 days

€415.00*

Key Features
  • Atto 542 conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF
  • Reacts with dsRed1, dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato

Product description
Quantity 200 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Atto 542
Application ICC/IF
Purification Affinity chromatography
Reactivity dsRed1/dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, mTagBFP or their most common derivatives
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 2B12
Immunogen RFP
Formulation 5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:1,000
Background

protag-HiRes anti-RFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes RFP and its most common derivatives with high affinity and specificity. It recognizes mOrange2, dsRed1, dsRed2, tdTomato, mRFP, mCherry, mScarlet-i.

In protag-HiRes anti-RFP-X1, each fluorophore is coupled to exactly one single-domain antibody, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Downloads
File
Category
Size
Filetype
File ICC/IF
Category Protocol
Size 805.06 KB
Filetype pdf
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