protag-HiRes anti-RFP-X4 Atto 542

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Cat. No. 82406L

Available, delivery time 1-7 days

€415.00*

Key Features
  • Atto 542 conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF
  • Reacts with dsRed1, dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato

Product description
Quantity 200 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Atto 542
Application ICC/IF
Purification Affinity chromatography
Reactivity dsRed1/dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, mTagBFP or their most common derivatives
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 2B12/ 2A1
Immunogen RFP
Formulation 1.25 µM of each fluorescently labeled single-domain antibody clone in buffered saline, 50% glycerol, 0.09% sodium azide; 5 µM total fluorophore concentration
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:250
Background

protag-HiRes anti-RFP is a blend of two camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It is ideal to detect mRFP and mCherry.

In protag-HiRes anti-RFP-X4, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. The blend of two camelid single-domain antibodies therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our protag single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our protag-HiRes anti-RFP-X4 can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our protag-HiRes anti-RFP-X4 products excellent tools for all microscopy techniques.

Downloads
File
Category
Size
Filetype
File ICC/IF
Category Protocol
Size 805.06 KB
Filetype pdf
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