protag-HiRes anti-TagFP-X2 Alexa 647

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Cat. No. 83212L

Available, delivery time 1-7 days

€415.00*

Key Features
  • Alex 647 conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF
  • Reacts with mKate, mKate2, mTagBFP, mTagRFP, mTagRFP657

Product description
Quantity 200 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Alexa 647
Application ICC/IF
Purification Affinity chromatography
Reactivity mKate/mKate2, mTagBFP, mTagRFP, mTagRFP657
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, dsRed or their most common derivatives
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 1H7
Immunogen TagFP
Formulation 2.5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500
Background

protag-HiRes anti-TagFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes fluorescent proteins derived from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657, mKate and mKate2 with high affinity and specificity.

In protag-HiRes anti-TagFP-X2, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. protag-HiRes anti-TagFP-X2 can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, the protag-HiRes anti-TagFP-X2 can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - render the protag-HiRes anti-TagFP-X2 products superior tools for all microscopy techniques.

Downloads
File
Category
Size
Filetype
File ICC/IF
Category Protocol
Size 805.06 KB
Filetype pdf
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.