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anti-Perilipin 5 (N-terminus) guinea pig polyclonal, serum

Polypeptide reacting: MLDP = myocardial lipid droplet protein (also described as OXPAT/PAT-1/Perilipin 5). MLDP pertains to the PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologuous domain called PAT. Additional PAT proteins (perilipins 1 - 5) including adipophilin (ADRP/perilipin 2), perilipin (perilipin 1), and TIP47 (perilipin 3) are expressed in differentiation-related stages of lipid metabolism.
Cat. No.: GP44
Quantity:  100 µL

Delivery Time: usually 1-7 working days

$333.00
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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptides (N-terminal aa 1-17/16-36 of human MLDP)
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100 - 1:200

Background

Details

Polypeptide reacting: MLDP = myocardial lipid droplet protein (also described as OXPAT/PAT-1 and perilipin 5 or PLIN5), MW 50,844 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE).
MLDP / PLIN5 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologuous domain called PAT.
Additional PLIN/PAT proteins include adipophilin (ADRP, PLIN2), perilipin (PLIN1), and TIP47 (PLIN3) which are expressed in differentiation-related stages of lipid metabolism.

No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.

Tissue localization: MLDP/PLIN5 is positively detected in heart and skeletal muscle.

Reactivity on cultured cell lines: HaCat (human), SV80 (human).

Reference

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