- Mouse monoclonal
- Suitable for ELISA, IHC and WB
- Reacts with human
- Isotype: IgG2b
Product description
| Quantity | 100 µg |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG2b |
| Conjugate | Unconjugated |
| Application | ELISA, IHC, WB |
| Purification | Affinity chromatography |
| Reactivity | Human |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | 23-3-4 |
| Immunogen | Recombinant protein |
| Concentration | 100 µg/ml after reconstitution with 1 ml dist. water |
| Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - paraffin | 1:300-1:1,000 (microwave treatment recommended) |
| ELISA | 1:50 |
| Western Blot (WB) | 1:1,000 |
Background
Calprotectin is a functional heterocomplex of small EF hand calcium binding proteins S100A8 and S100A9. The heterocomplex is expressed in various cell types and tissues and induced upon inflammation. The complex is released by neutrophilic granulocytes in inflammatory disorders such as inflammatory bowel disease and rheumatoid arthritis. Released Calprotectin serves as a leukocyte chemoattractant and modulates inflammation via damage receptors. Via its binding to the receptor of advanced glycation end-products (RAGE), calprotectin is implicated in chronic inflammation and cancer formation.
Tissue expression: strong expression in myeloid cells and psoriatic keratinocytes.
The antibody recognizes both the S100A8/A9 heterocomplex as well as S100A9 alone but not S100A8.
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
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