Using Centrifugation

OptiPrep™
Nycodenz®
LymphoPrep™
PolymorphPrep™


Much of modern cell and molecular biology relies on the isolation of pure, functionally intact populations of cells, subcellular organelles and macromolecules in high yield and both research and clinical applications provide an impetus for the development of improved separation technology. The media are used in density gradient separation for the purification not only of cells and organelles but also membrane vesicles, viruses, proteins, nucleic acids and lipoproteins.

Our density gradient media developed by Axis Shield are:

  • Proven to be non-toxic,
  • Have a low osmolality
  • Are sufficiently dense to permit the purification of all types of biological particles
  • In compliance with FDA and EU cGMP standards and regulatory requirements. 

OptiPrep™


OptiPrep™ is a ready-made, sterile and endotoxin-tested solution of iodixanol. Suitable for the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendritic cells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes, endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients. Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.



View Product

Nycodenz®


Freely soluble in water up to 80% (w/v). Suitable for the the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendritic cells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes, endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients. Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.


View Product

LymphoPrep™


LymphoPrep™ Tube is a sterile tube in which the LymphoPrep™ is contained below a plastic filter disc. Especially useful for the separation of monocytes (monocytes and lymphocytes) from whole blood.


View Product

PolymorphPrep™


PolymorphPrep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human polymorphonuclear leucocytes (granulocytes) from whole blood.


View Product

All Density Gradient Media Products

OptiPrep™ (Iodixanol)
Cat. No. 1114542
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

€175.00*
Nycodenz®
Cat. No. 1002424
  • Freely soluble in water up to 80% (w/v)
  • Suitable for the the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.

€462.00*
LymphoPrep™ (6x500 mL)
Cat. No. 1114547_1

Variants from €85.00*
€366.00*
PolymorphPrep™
Cat. No. 1114683
  • Ready-made, sterile and endotoxin tested solution
  • Suitable  for the isolation of human polymorphonuclear leucocytes (granulocytes) from whole blood

€110.00*

Know what you need?

Login and order directly
from your account.

Order Now

Need more information?

Visit our support hub for FAQs,
references and downloads.

Visit Support Hub